Nikon Eclipse TS100 User Manual

6 Troubleshooting
1 Optical
E-39
Poor image quality (No effect of phase contrast; poor
contrast or resolution)
Causes Corrective Measures
(For phase contrast microscopy:)
No annular diaphragm is in optical path.
Use an annular diaphragm that matches
the objective. (P.18)
(For phase contrast microscopy:)
Ph codes of objective and annular
diaphragm do not match.
Use an objective and annular diaphragm
having the same Ph code.
(For phase contrast microscopy:)
Annular diaphragm image is not
centered on phase ring.
Center the annular diaphragm. (P.18)
(For phase contrast microscopy:)
Aperture diaphragm is closed.
Fully open the aperture diaphragm.
(P.17)
(For NAMC microscopy:)
No NAMC objective is in optical path.
Place an NAMC objective in optical path.
(For NAMC microscopy:)
NAMC codes of objective and slit
diaphragm do not match.
Use an objective and slit diaphragm
having the same NAMC code.
(For NAMC microscopy:)
Slit diaphragm image is not adjusted to
be contained in G area of modulator.
Adjust the position of the slit diaphragm.
(P.20)
Condenser position is wrong.
Check for proper attachment to
illumination pillar.
A specimen of culture container bottom
thickness (slide glass thickness) or cover
glass thickness specified on the
objective is not used. Or the specimen is
upside down.
Place a culture container (slide glass)
thickness of around 1.2 mm and cover
glass thickness of 0.17 mm. Place a
specimen of the specified thickness faces
the objective. (P.27)
Correction ring of objective is not in
right position (when using objectives
with correction rings).
Adjust the correction ring to match
container thickness. (P.27)
Lens is dirty. (Condenser, objective, or
eyepiece)
Clean the lens. (P.46)
No immersion oil is applied to the tip of
an oil-immersion objective.
Apply the Nikon immersion oil. (P.27)
The Nikon immersion oil is not used.
Use the Nikon immersion oil.
The immersion oil contains air bubbles.
Remove the air bubbles. (P.28)
Immersion oil adhering to the tip of the
dry-type objective.
(especially, 40x objective.)
Clean it as appropriate. (P.28)

Nikon Eclipse TS100 Specifications

General

Type	Inverted Microscope
Optical System	CFI60 infinity optical system
Power Supply	AC100-240V, 50/60Hz
Illumination	6V/30W halogen lamp
Eyepiece	10X
Objectives	4x, 10x, 20x, 40x
Observation Methods	Brightfield
Focusing	Coarse and fine focusing
Condenser	Condenser N.A. 0.3
Magnification	40x to 400x