Ocean Optics QE Pro - Appendix A: Calibrating the Wavelength of the QE Pro; Overview and Calibration Basics

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Appendix A
Calibrating the Wavelength of
the QE Pro
Overview
This appendix describes how to calibrate the wavelength of your spectrometer using SpectraSuite
spectrometer operating software. Though each spectrometer is calibrated before it leaves Ocean Optics,
the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions.
Ocean Optics recommends periodically recalibrating the QE Pro.
About Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:

p
= I + C
1
p + C
2
p
2
+ C
3
p
3
Where:
 = the wavelength of pixel p
I = the wavelength of pixel 0
C
1
= the first coefficient (nm/pixel)
C
2
= the second coefficient (nm/pixel
2
)
C
3
= the third coefficient (nm/pixel
3
)
You will be calculating the value for I and the three Cs.