Sample Experiments
USB2000 Operating Instructions 23
6. Place a sample of the solvent into a cuvette and take a reference spectrum. You must take a reference
spectrum before measuring transmission.
Note: Do not put the sample itself in the path when taking a reference spectrum, only the solvent.
Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference from the
menu bar to store the reference. This command merely stores a reference spectrum in memory. You
must select File | Save | Reference from the menu bar to permanently save the spectrum to disk.
3. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the Store Dark
Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This command
merely stores a dark spectrum in memory. You must select File | Save | Dark from the menu to
permanently save the spectrum to disk.
Note: If possible, do not turn off the light source when taking a dark spectrum. If you must turn off your
light source to store a dark spectrum, allow enough time for the lamp to warm up again before
continuing your experiment.
You must take a dark spectrum before measuring transmission.
4. Put the sample in place and verify that the light path is clear. Then, take a transmission measurement by
clicking the Transmission Mode icon on the toolbar or selecting Spectrum | Transmission Mode from
the menu bar. To save the spectrum to disk, click the Save icon on the toolbar or select File | Save |
Processed from the menu bar.
Note: If you change any sampling variable (integration time, averaging, smoothing, angle, temperature,
fiber size, etc.), you must store a new dark and reference spectrum.
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