stacked on top show the distribution of dead cells.
Overall larger cells indicate an older yeast
population, as the cells grow larger with each
budding (splitting).
To review the image analysis, click on one of the results to
enlarge the image (Fig. 21). Only the yeast inside the circle is
analyzed. The analysis also includes the cells touching white
parts of the circle in the analysis, and ignores cells touching the
red areas.
This is similar to determining concentration with a
counting chamber; during this process,
concentration is determined accurately by omitting
cells that touch the upper or left edge of the
counting grid.
Recognized cells are encircled and marked in color. Depending
on the selected method, the analysis is able to differentiate
between live, dead and dividing (budding) cells.
Budding cells are handled according to the recommendation of
leading experts on the field of yeast management.
Any budding cell (mother and daughter) is ignored within the
viability calculations. Budding cells cannot metabolize methylene
blue or violet as efficiently, thus they are often colored blue, even
though they are viable and reproducing.
They are only included in the concentration calculation if they are
at least half the size of the mother cell. Smaller buds are ignored.
In summary, the recognition works as follows:
22
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