Recipe: Alkaline Methylene Blue 0.01%
Alkaline methylene blue solution – 100 ml
Chemicals:
• Methylene blue (Sigma)
• 0.1M Glycine buffer (pH 10.6)
• Distilled water
Procedure:
• Dissolve 0.1g methylene blue in 100 ml distilled water (0.1% MB stock solution)
• Mix 10 ml of the MB stock solution with 90 ml of the 0.1M glycine buffer. This solution is
used for the viability staining.
0.1M Glycine buffer (pH 10.6) – 100 ml
Chemicals:
• 1M NaOH solution (Merck)
• Glycine (Sigma)
• Distilled water
Procedure:
• Dissolve 0.75 g glycine in 70 ml water
• Adjust pH with the NaOH solution to 10.6
• Fill volume to 100 ml with distilled water
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