Olympus FV1000 Guidelines

Olympus FV1000 confocal microscope
Please
• read and follow our guidelines.
• don´t use without introduction by ALMF staff
• Only switch on parts you need for your purposes
(e.g. if you don’t need the Hg lamp for epifluorescence like for overnight on confocal
microscopes)
• report problems to us without delay
Laserlines:
Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
Red Diode Laser: 635 nm (Cy5, Alexa 633/647)
Switching on the system:
•
Switch on the PC (button next to floppy drive).
• Switch on the microscope components with the power manager (Mic-
control, PSU1+2, HBO lamp if neccessary)
• Switch on the lasers:
A) Laser combiner (FV10-MCPSU)
B) 559 nm laser if needed
1) Main switch
2) Wait until 'TEMP' is not blinking anymore
3) Turn key to 'on' position

Olympus FV1000 Specifications

General

Type	Confocal Laser Scanning Microscope
Image Resolution	Up to 4096 x 4096 pixels
Time-Lapse	Yes
Detectors	PMT (Photomultiplier Tubes)
Objective Lenses	Compatible with a wide range of Olympus objectives
Software	Fluoview FV10-ASW software
Scanning Method	Galvanometer
Z-Stack	Yes
Spectral Detection	Yes, with spectral detector option
Weight	Varies depending on configuration
Laser Sources	Multi-line Ar laser (458, 488, 515nm), HeNe(G) laser (543nm), HeNe(R) laser (633nm)