Olympus FV1000 confocal microscope
Please
• read and follow our guidelines.
• don´t use without introduction by ALMF staff
• Only switch on parts you need for your purposes
(e.g. if you don’t need the Hg lamp for epifluorescence like for overnight on confocal
microscopes)
• report problems to us without delay
Laserlines:
Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
Red Diode Laser: 635 nm (Cy5, Alexa 633/647)
Switching on the system:
•
Switch on the PC (button next to floppy drive).
• Switch on the microscope components with the power manager (Mic-
control, PSU1+2, HBO lamp if neccessary)
• Switch on the lasers:
A) Laser combiner (FV10-MCPSU)
B) 559 nm laser if needed
1) Main switch
2) Wait until 'TEMP' is not blinking anymore
3) Turn key to 'on' position
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