Quick Start Guide
Start-Up 5
Shut-Down 5
............................................................................................................Quick Guide to Getting an Image 6
........................................................................................................Place the sample on the microscope 7
........................................................................................Finding the sample – transmitted light or DIC 7
..................................................................................................................................Brightfield vs. DIC 9
....................................................................................................Finding the sample – epi-fluorescence 9
................................................................................................................Finding the sample - confocal 10
......................................................................................................Setting Up for Confocal Microscopy 11
...................................................................................................................................Choosing the Dye 11
..............................................................................................................Changing the Dye Selection 12
...............................................................................................................Confocal acquisition controls. 12
.................................................................................................Settings for the fluorescence detectors. 13
.................................................................................................................Additional detector controls. 13
.......................................................................................................................................Laser Controls. 14
...................................................................................................................................Line Selection. 14
..............................................................................................................Line availability, Figure 7.2. 14
......................................................................................................Laser Power Selector, Figure 7.3. 15
.......................................................................................................................Scroll List, Figure 7.4. 15
....................................................................................................................Line Settings, Figure 7.5 15
.........................................................................................................................................Scan Controls 16
...........................................................................................................................Scan Mode, Figure 6.1 16
..........................................................................................................................Dwell Time, Figure 6.2 16
..................................................................................................................Scan Regions, Figure 6.3. 17
........................................................................................................Image Aspect Ratio, Figure 6.4. 17
.....................................................................................................................................Box Size, 6.5. 17
.............................................................................................................Box Size effect on resolution 18
......................................................................................................................................AutoHV, 6.6. 18
................................................................................................................................Scan Area Controls 18
..................................................................................................................................Field of View, 8.1. 18
.......................................................................................................................................Scan Area, 8.2. 18
.................................................................................................................................Scan Rotation, 8.3. 18
.....................................................................................................................................Scan Zoom, 8.4. 19
..........................................................................................................................Reset Scan Values, 8.5. 19
.....................................................................................................................................Scan Offset, 8.6. 19
..............................................................................Use of Sequential, Simultaneous and Averaging. 21
................................................................................Simultaneous Capture versus Sequential Capture. 21
....................................................................................................Two Methods for Sequential Capture 21
..................................................................................................Sequential Line Capture, Figure 9.1 21
...............................................................................................Sequential Frame Capture, Figure 9.1 21
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 1
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