Olympus Fluoview-1000 - Page 2

.....................................................................................................Scan mode and sequential capture 22
.......................................................................................................Using Channel Groups, Figure 9A. 22
....................................................................................Adjusting the channels with sequential imaging 22
...............................................................................Adjusting the channels with simultaneous imaging 22
...............................................................................................................................Collecting a Z-Series. 23
...........................................................................................Determine the depth of the optical volume. 23
..........................................................................................................Which way does the focus move? 23
..............................................................................................................Settings for z-series collection. 25
........................................................................................................................What is axial resolution? 25
...................................................................................................................................Vertical Sections. 26
...............................................................................................................Files: Saving and Transferring 26
...........................................................................................................................................Saving Files 26
.............................................................................................................The Olympus “OIB” format. 26
..............................................................................................................The Olympus “OIF” format. 26
.............................................................................................Where are Your Files on the Confocal? 27
....................................................................................................................................Export file types. 27
................................................................................................................Add a scale bar to your file 27
...............................................................................................................................Opening your Files. 27
.......................................................................................................................Olympus free file viewer 28
................................................................................................................Offline Fluoview workstation 28
.............................................................................................Importing Olympus datasets into ImageJ. 28
............................................................................................................Loci-tools Plugin for ImageJ. 28
...........................................................................................................Import Channel Order Macro. 28
...............................................................................................................................Image 5D plugin. 28
..........................................................................................File Import from the OIF.Files directory. 29
..........................................................................................................................................File Transfer. 29
.................................................................File transfer to Mac Pro ‘Fluoview’, confocal file server. 29
.....................................................................................Saving files directly to Mac Pro ‘Fluoview’. 29
..........................................................................................USB and Firewire Ports on the Mac Pro. 29
................................................................Recording to CD-ROM and DVD-ROM on the Mac Pro. 30
............................................Networked file transfer to remote servers from the Fluoview Mac Pro 30
....................................................................................................Using FTP to Windows computers 30
........................................................................Remote access to the Mac Pro from your laboratory. 30
..................................................................................................................Optimizing Image Collection 31
..............................................................................................Qualities of an Optimal Confocal Image. 31
...............................................................................................Overview of image capture properties 31
.........................................................................................................Overview of basic image capture. 31
.............................................................................................Basic settings for confocal microscopy 31
.................................................................................................................................Labeling Controls. 32
...............................................................................................................................Negative controls 32
................................................................................................................................Positive controls 32
............................................................................................................................The confocal aperture 32
........................................................................................................................Optimal Iris Diameter 32
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 2