Olympus Fluoview-1000 User Manual

1.1 Place the sample on the microscope
1. Make sure the objective lens is not extending above the level of the specimen holder;
a. If it is, rotate the focus knob to lower the objective lens (set to Coarse Focus)
2. Press ‘ESC’ button behind either focus knob to lower the objectives;
3. Move the condenser up, out of your way;
4. Install the appropriate specimen holder in the stage, if needed;
5. Place the slide on the stage with the coverslip downwards;
6. The sample should be centered in the stage opening to avoid bumping the sample holder with the
lens as you move the stage to position the desired field of interest;
7. Press ‘ESC’ button to return the lens to focus;
8. Lower the condenser back into position;
9. Do not use the keypad on the front of the microscope, it has been disabled;
10. All microscope control must be through the Fluoview software windows, Figures 2, 3;
11. Open the Microscope Controller window, if it is not open: Fluoview
menu>Device>Microscope Controller…, Figure 2.1;
12. Select an objective: Microscope Controller Window>Objective Lens>click on desired lens,
Figures 2.4 and 3.2, see Table 2;
13. Condenser setting: Microscope Controller Window>Condenser sets condenser automatically –
don’t touch, Fig. 3.1;
14. Select mirrors (fluorescence filter cubes): Microscope Controller Window>Mirror>click on
desired mirror, Fig. 3.3, see Table 1;
15. Fine focus/coarse focus: press the ‘F/C’ button below each focus knob, Figure 12.2, to toggle
between focus modes;
16. Lower/raise lens to apply immersion oil: press the ‘ESC’ button behind each focus knob, Figure
12.3;
17. Center the region of interest in the field of view with the joystick;
1.2 Finding the sample – transmitted light or DIC
1. Center the sample over the stage opening to avoid bumping the stage with the lens;
2. Refer to Microscope Controls, Sections 7.2 and 7.3, for details on using DIC and setting Koehler
illumination;
3. Open the Microscope Controller window, Fluoview menu>Device>Microscope Controller…,
Figure 2.1;
4. All microscope control must be through the Fluoview software windows, Figures 2.1, 2.4;
a. Select lens: Microscope Controller Window>Objective Lens, Figure 3.2, Table 2;
b. Condenser controls are set automatically: Microscope Controller Window>Condenser –
don’t touch, Fig. 3.1;
c. DIC condenser settings are automatically selected when transmitted lamp is on:
Microscope Controller Window>Mirror>Select DIC (DICT), Fig. 3.3;
5. Turn on the transmitted light;
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 7

Olympus Fluoview-1000 Specifications

General

Brand	Olympus
Model	Fluoview-1000
Category	Microscope
Language	English