Olympus Fluoview-1000 - Figure 12. DIC and Focus Controls, Lower Half of the Microscope

Theoretical lateral resolution:
€
wavelength(nm)
2(NA)
Wavelength = emission, in nanometers, usually the central wavelength of the emission band of the
barrier filter.
NA = numerical aperture of the objective lens.
Examples:
Using a 20X/NA0.75 objective with the green channel, 540 nm/2X.75 = 540 nm/1.5 = 360 nm lateral
resolution (0.36 µm).
Zoom factor for 2X oversampling = Pixel size
(2)
(wavelength)(NA)
Figure 12. DIC and Focus Controls, Lower Half of the Microscope
12.1 Focus Knob
12.4 DIC Analyzer slider
(out position)
12.3 Lens
Drop/Raise
12.6 DIC Analyzer
Adjustment knob
12.5 DIC Analyzer
Locking screw
12.7 Manual shutter
(open position)
12.8 Current mirror position
12.2 F/C Coarse/Fine Focus
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 40