Figure 5. Channel Settings for the Photomultiplier Tube (PMT)
2.5 Laser Controls.
Each fluorophore selected in the Dye List will select an appropriate laser line and some default intensity
setting. The Line Setting values are given as a percentage of full rated output of the laser (mW) entering
the scanhead. You can change the power setting for each line or turn lines off to assess bleedthrough
(details of bleedthrough are in section 6).
2.5.1 Line Selection.
Active lines are indicated by a check mark, Figure 7.1. Clicking in these boxes will toggle lines
between active and blocked. This control blanks the lines in the scanhead, it does not turn the laser itself
on or off.
2.5.2 Line availability, Figure 7.2.
Laser lines associated with each set of controls are listed in this column. This list is not affected by a
whether or not a laser has been powered up.
5.6 Channel
5.2 Amplifier Gain
5.1 PMT Voltage
5.5 Channel group
5.7 Active if
checked
5.3 Offset (black level)
5.4 Laser Settings
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011 14
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