Olympus Fluoview FV1000 - User Manual

540 pages

Save Page as PDF

To Next Page

User’s Manual

FLUOVIEW

FV1000

CONFOCAL LASER SCANNING

BIOLOGICAL MICROSCOPE

[OPERATION] FV10-SW Ver 5.0c

This user’s manual is for the software to be run on Olympus FLUOVIEW FV1000 Confocal Laser

Scanning Biological Microscope. To ensure safety, obtain optimum performance and familiarize

yourself fully with this product, we recommend that you study this manual thoroughly before operation.

This user’s manual is composed of two volumes including “OPERATION INSTRUCTIONS” and

“MAINTENANCE”. Together with this manual, please also read the “SAFETY GUIDE”, “HARDWARE

GUIDE” of User’s manual FLUOVIEW FV1000 and the instruction manual of the microscope in order to

understand overall operation methods. To ensure the safety operation of laser system, we recommend

you to study the manual of each laser and the light source equipment besides this manual.

Retain this manual in an easily accessible place near a system for future reference.

Petition

X7274

Main Page

Other manuals for Olympus Fluoview FV1000

Overview28 pages

Questions and Answers

L
Lisa BeckerAug 15, 2025
What to do if the laser is not output from the extremity of the Olympus Fluoview FV1000 Microscope objective?
- J
  Joshua PhamAug 15, 2025
  If the laser is not output from the extremity of the objective, there could be several reasons. First, ensure that the laser unit is turned on and the emission key is set to ON. Next, verify that the correct laser wavelength is selected. For manual systems, check that the manual shutter of the fluorescence mirror unit is open and that the reflective mirror inside the fluorescence mirror unit is correctly positioned in the light path. Also, make sure the objective is in the light path, especially ensuring it's in the click position if using a manual revolving nosepiece. If the laser beam is too weak, increase the laser intensity. Lastly, confirm that the properties of the combined cube unit used for the ASU or OPD match the selected laser wavelength.
F
Francis CampbellOct 30, 2025
Why can't I observe a fluorescence image with my Olympus Fluoview FV1000?
- O
  oclarkOct 30, 2025
  If you cannot observe a fluorescence image, consider the following potential causes. The confocal pinhole diameter might be too small, so try increasing it. Ensure that the excitation Dichroic Mirror selection matches both the observed fluorescence wavelength and the excitation laser wavelength. Also, verify that the spectral dichroic mirror and barrier filter selections match the observed fluorescence wavelength. If you are using a spectral detection system, confirm that the acquisition wavelength region setting is suitable for the observed fluorescence wavelength. Check that the fluorescent dyeing method and excitation wavelength are compatible, selecting a laser optimum for the fluorescent dyeing method. Ensure that the focus is properly adjusted and that the PMT voltage of the detector...