Getting Started FLUOVIEW/Outline of LSM Observation Procedures
OPERATION INSTRUCTIONS
1-9
Page
1-2 Outline of LSM Observation Procedures
• Fluorescence observation procedure
If no image is
displayed
Set the observation condition.
If an image
is displayed
Start the system.
•
Turn the system power ON.
(Section 1-2-1)
•
Start the FLUOVIEW software.
(Section 1-2-1)
•
Set the multiple sections to be
observed.
(Section 1-2-7-7)
•
Set the area to be observed.
(Section 1-2-7-8)
• Set a lower scan speed.
(Section 1-2-7-9)
•
Stop repeated scanning.
(Section 1-2-7-10)
z
Select the LSM light path.
z
Let the mirror cube into the
light path.
(Section 1-2-3)
When the combination using the laser
combiner is used, set the optimum ND
filters for the laser.
(Section 1-2-6)
z
Let an appropriate cube for
dyeing method into the light
path.
z
Select the light path for 100
binocular tube and focus on
the specimen.
(Section 1-2-2)
Adjust PMT Voltage.
(Section 2-1-1-4-9)
Change the ND filter with a filter
with higher transmittance
(Section 1-2-6)
Set the objective magnification.
(Section 1-2-7-1)
•
Set the zoom ratio to 1X.
(Section 1-2-7-2)
•
Set the channels.
(Section 1-2-7-3)
•
Set the highest scan speed.
(Section 1-2-7-4)
•
Select the XY observation mode.
(Section 1-2-7-5)
•
Perform repeated scanning.
(Section 1-2-7-6)
Image is
displayed in the
[Live] panel of
the software
Set the dyeing method.
(Section 1-2-41-2-5)
Acquire an image.
(Sec 1-2-8)
Save the image.
(Sec 1-2-9)
Exit from the FLUOVIEW software.
(Sec 1-2-10)
Turn the system power OFF.
(Sec 1-2-10)
If the image is still not displayed
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