APPLIED OPERATIONS/Image Acquisition
OPERATION INSTRUCTIONS
2-64
Page
2-2-3-2 Dual-Fluorochrome Image
The wavelength obtained by dual-fluorochrome dyeing can be acquired and observed as
images of the channels (Ch1 or Ch2).
The subsequent description deals with the differences in operation between the
monochrome dyeing and dual-fluorochrome dyeing.
z Set the two or more dyeing methods.
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Dyes] sub-
panel.
Fig. 2-21 [Dyes] Sub-panel
2. Select the specimen dyeing method by dragging desired dye names in the [Available
Dyes] list box in the [Selected Dyes] group box to the field immediately above the list
box.
3.
Click the <Apply> button to apply the selected dyeing method to the [Ch] group box on
the upper part of the [Acquire] panel.
When the dyeing method is selected from the [Available Dyes] list box and the
<Apply> button is clicked, a channel for acquiring fluorescence is set
automatically according to the changed filter. And the dyeing method is shown
in the [Ch] group box.
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Prev.> button
Sets the dyeing method which was
set last time by clicking the
<Apply> button.
<Apply> button
pplies the dyeing method
dragged in the [Selected
Dyes] group box to the
[Ch] group in the [Acquire]
panel.
Place the pointer on the icon displayed
in the [Selected Dyes], and the dyeing
method is shown in the pop-up display.
TIP
[Assign dyes manually]
check box
Checking this enables the
manual setting. Dragging
the dyeing method in the
list directly to the [Ch]
group box assigns the dye
to the desired channel.
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