Olympus Fluoview FV1000 - Confocal Aperture Setting

Setting the Confocal Aperture/Confocal Aperture Setting
MAINTENANCE
3-5
Page
If the message below appears by clicking the <C.A. Adjustment>
button, exit from the FLUOVIEW software and
execute “FVCAReader.exe.” in the “FLUOVIEW” folder under drive C.
3-3 Confocal Aperture Setting
1. Engage the 10X or 20X objective in the light path and place the mirror specimen
on the stage. (Place a fluorescence specimen if you want to perform adjustment
using a fluorescence specimen.)
2. In the [Laser Unit] group box in the [Optical System Configuration] window, select
the wavelength of the laser used in confocal aperture setting (488 nm). (Select
the excitation wavelength if you want adjustment using a fluorescence specimen.)
NOTE