Olympus Fluoview FV1000 - Brightness Overlap Level between 2 Channels (Colocalization)

APPLIED OPERATIONS/Image Processing
OPERATION INSTRUCTIONS
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2-6-4 Brightness overlap level between 2 channels (Colocalization)
This function enables to observe overlap intensity of two channel of image.
1. Acquires or opens images which more than two channels, and displays the images on
image window.
2. On the image window, displays and overlaps 2 channel image that to be observed. See
section 2-4-4-2 for overlapping the image display.
3. On the overlapped images, click right-mouse button to show the following pop up menu.
4. Select [Install Colocalization: ∗∗∗∗.v ∗∗∗∗] of [ViewProcessor]. Then [Colocalization
Processor] appears.
[∗∗∗∗] shows the dyeing method or its number of channel.
There are the following 4 Annotation Mode in [Colocalization Processor] dialog box.
• None Mode
• Thresholds Mode
• Min-Max Bounds Mode
• Regions Mode