APPLIED OPERATIONS/Image Processing
OPERATION INSTRUCTIONS
2-247
Page
2-6-4-1 Annotation Mode
1 None Mode
None Mode is the default of [Colocalization Processor] dialog box. The mode can be
changed by option button in [Annotation Mode] Group Box. The items described for this
mode can be used in other modes.
Fig. 2-96 [Colocalization Processor] Dialog Box
In case of Annotation Mode is [None]
.
[Data Selection] Group Box
<Disabled> Option Button
Graph and Text box will disappear from
[Colocalization Processor] dialog box.
<Current slice> Option Button
This button executes colocaliztion fo
images displayed on image window.
[Histogram] Graph
It acquires brightness value of 1st channel
selected on horizontal axis and brightness o
value of other channel on vertical axis and
displays brightness distribution. The point with
the value which less than [LUT min] text box is
colored with blue, and the point more than
[LUT max] value is colored with in red; and
other point will be colored by gray scale.
[Summary] Text Box
Summary : Dye method of vertical and
horizontal axis or channel number
Mode : Option button selected at [Data
Selection] group box
Samples processed : Total numbers o
pixels on whole image to be processed
Binning : [Bin Width] Setting condition o
text box
Max. samples in any bin : Max. value in
plot data on graph
[Annotation Mode] Group Box
Select either [None], [Threshold], [Min-
Max Bounds] or [Regions] option button.
In case of [Threshold], [Min-Max Bounds]
or [Regions] are selected, text box
appears in [Annotation] Group Box.
[Colocalization Processor] is displayed,
[None] is selected.
<Accept Histogram> Button
[Histogram] graph appears in image
window.
The graph can be handled same with the
acquired image.
Some measuring results indicated may
have the computation error while the
software process.
[LUT Max] Text Box
Double-click inside text
box so that the display
can alternately be
changed in two methods.
1. All data uniform display
2. Binary display of data
presence
[LUT Min] Text Box
<Hide> Button
It hides [Colocalization
Processor] dialog box.
The [Colocalization
Processor] dialog box
appears again by pressing
<Colocalization
Processor> button in image
window.
[Zoom] Text Box
It sets display
magnification of
[Histogram] graph.
[Bin Width] Text Box
It sets resolution of
[Histogram] graph. For
example, if the value is
16, it displays
brightness in 16 gray-
scale as one plot.
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