Olympus Fluoview FV1000 - Selecting the Filters

Getting Started FLUOVIEW/Outline of LSM Observation Procedures
OPERATION INSTRUCTIONS
1-23
Page
1-2-5 Selecting the Filters
The excitation dichroic mirror, beam splitter and barrier filters are set automatically to the light path according to the dyeing method
selected for the specimen.
To change filters, see section 1-3-2-4, “Configuring the Filters” in this volume and follow instructions in the [Optical System
Configuration] window.
The following table shows the possible combinations of the barrier and excitation filters.
Laser Combination
Excitation
Dichroic Mirror
Beam
Splitter 1
Beam
Splitter 2
Beam
Splitter 3
Barrier Filter 1 Barrier Filter 2
Barrier
Filter 3
Barrier
Filter 4
Multiline Ar. HeNe(G),
HeNe(R)
458/488/515, 543, 633
(3 channels)
(1) BS20/80
(2) DM405/488
(3) DM488/543/633
(4) DM458/515
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM560
(4) SDM510
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM640
(4) -
(5) -
(6) -
(1) BA505IF
(2) BA505-525
(3) BA480-495
(4) -
(5) -
(6) -
(1) BA560IF
(2) BA560-620
(3) BA535-565
(4) -
(5) -
(6) -
(1) BA650IF
(2) -
(3) -
(4) -
(5) -
(6) -
Multiline Ar. HeNe(G),
HeNe(R)
458/488/515, 543, 633
(3 channels)
SPD system
(1) BS20/80
(2) DM405/488
(3) DM488/543/633
(4) DM458/515
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM560
(4) SDM510
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM640
(4) -
(5) -
(6) -
(1) BA505IF
(2) BA505-525
(3) BA480-495
(4) -
(5) -
(6) -
(1) BA560IF
(2) BA560-620
(3) BA535-565
(4) -
(5) -
(6) -
(1) BA650IF
(2) BA560IF
(3) -
(4) -
(5) -
(6) -
Multiline Ar,
Kr, HeNe(R)
458/488/515, 568, 633
(3 channels)
(1) BS20/80
(2) DM405/488
(3) DM488/568/633
(4) DM458/515
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM560
(4) SDM510
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM640
(4) -
(5) -
(6) -
(1) BA505IF
(2) BA505-550
(3) BA480-495
(4) -
(5) -
(6) -
(1) BA585IF
(2) BA585-615
(3) BA535-565
(4) -
(5) -
(6) -
(1) BA650IF
(2) -
(3) -
(4) -
(5) -
(6) -
Multiline Ar,
Kr, HeNe(R)
458/488/515, 568, 633
(3 channels)
SPD system
(1) BS20/80
(2) DM405/488
(3) DM488/568/633
(4) DM458/515
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM560
(4) SDM510
(5) -
(6) -
(1) Mirror
(2) Glass
(3) SDM640
(4) -
(5) -
(6) -
(1) BA505IF
(2) BA505-550
(3) BA480-495
(4) -
(5) -
(6) -
(1) BA585IF
(2) BA585-615
(3) BA535-565
(4) -
(5) -
(6) -
(1) BA650IF
(2) BA560IF
(3) -
(4) -
(5) -
(6) -