Getting Started FLUOVIEW/Outline of LSM Observation Procedures
OPERATION INSTRUCTIONS
1-18
Page
1-2-3-2 Combination with Inverted Microscope (IX81 FVF)
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Settings]
sub-panel, and .select the <LSM> button in the [Light Path] group box.
The <LSM> button looks pushed in to indicate that it is selected.
(When scanning is started while the <BI> button is selected, the LSM light path is
selected automatically. It is switched back to the visual observation automatically when
scanning completes.)
2. Push the hand switch button (7) to set reflected light fluorescence mirror unit to
1
.
3. When a fluorescence observation alone is required, disengage the U-DICTS
transmitted DIC slider (3) by setting the switch to the pulled-out position. When a
transmitted light DIC observation or a simultaneous fluorescence & transmitted light
DIC observation is required, engage the transmitted DIC slider (3) and the optimum
transmitted DIC slider for the objective into the light path by operating the universal
condenser (4).
In a simultaneous fluorescence & transmitted light differential interference observation,
leaving the transmitted DIC slider (3) within the light path degrades the fluorescence
image resolution to some extent.
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