Software Setup/Adding the filters
MAINTENANCE
1-24
Page
1-5 Adding the filters
The FLUOVIEW software allows the filters to be associated with dyeing methods.
As a result, specifying a dyeing method sets the detection channel and added filter
automatically.
This section assumes that a filter is added to the scanning unit and describes how to add the
new filter in the software and how to associate the added filter to a dyeing method by taking
the optional filter addition procedure as an example.
Example) To add “CFP” (which uses the LD440 laser) to the dyeing method and add
“DM440” (optional excitation dichroic mirror) and “BA465-495” (optional barrier filter) to
Channel 1 and detect “CPF” in Channel 1.
Addition Procedure Example
Set the dyeing method
(Section1-5-3)
Add a filter
(Section 1-5-1)
Set the database
(Section 1-5-4)
End the setup.
•
Setting the dyeing method
•
Setting the excitation dichroic mirror
•
Setting the beam splitter
•
Setting the barrier filter
•
Setting the detected channel
Set the scanning unit
(Section 1-5-2)
1-5-1 Adding Filters
For the method of adding filters to the scanning unit, please consult your local Olympus
representative for assistance.
In this example, let us add “DM440” to excitation dichroic mirror turret No. 5 and ”BA465-
495” to Channel 1 barrier filter No. 3.
To Next Page
Loading...
