APPLIED OPERATIONS/Merger/Extraction of Image Channels
OPERATION INSTRUCTIONS
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Page
3. Display the image slice to start the range at the front using the <Display> button at the
top of the image window.
4. Click the <Set start position> button. (If the start position is not set, image No. 0
becomes the start image automatically.)
5. Display the image slice to end the range at the front using the <Display> button.
6. Click the <Display> button. (If the end position is not set, image No. n-1, assuming the
number of image slices is n, becomes the start image automatically.)
2-14-2 Merging Image Channels
For instance, when a specimen is imaged with two scans for 2-channel fluorescence
observation and 1-channel transmitted light observation, the transmitted light image can be
merged to the fluorescence images to create a 3-channel image. Note that the display
gradation of the images obtained by overlaying 3 channels may be poor than original.
1. Open the two files to be used in creating a new image.
The image files used in overlay are subjected to the following restrictions.
· The sizes of the images in the two image files should be identical.
· The number of data bits in the two image files should be identical.
(For example, it is not possible to overlay a Fluoview Multi Tiff file with a
Single TIF(S) 8-bit file.)
When the image in one of the image files is composed of multiple image slices,
it is possible to use only some of the slices by setting a slice range. See section
2-14-1, “Setting the Range of Multiple Image Slices” for the operation
procedure.
When the images in both image files are composed of multiple image slices,
the overlaid image will have the same number of image slices as the file with
the fewer image slices.
Example 1: XYZ image + XY image = XY image
(The first image slice of the XYZ image is overlaid with the XY image.)
Example 2: XYZ image with 10 slices + XYZ image with 5 slices = XYZ image
with 5 slices
(The first five image slices are overlaid.)
NOTE
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