Olympus FV1000 - User Manual

OlympusFV1000Userguide August3,2011
Olympus FV1000 MPE Microscope User Guide
A.SystemStartUp
1. Signintothelogbook.Indicatethelaserlinesyouwilluse;visiblelaserandIRlaser.
2. Turnonmercurylamppowersupply
b. 
3. Turnonmicroscopecontroller andscanner.
Microscopecontroller‐switchON
c
Scanner–switchONandturnKeytoON
d.
4. Turnonlasercombiners
ef.
5. Turnonlaserpowersupplies
Multi‐Ar
g:SwitchtoONandturnKeytoON.
HeNe
h:TurnKeytoON.
6. Turnoncomputer
iifitisnoton.
7. Enterusername/passwordtologontoWindowsXP.
8. StarttheFV10‐ASWprogramandenter UserIDand
password.

b
c
d

e
f
g
i
h

Olympus FV1000 Specifications

General

Type	Confocal Laser Scanning Microscope
Image Resolution	Up to 4096 x 4096 pixels
Time-Lapse	Yes
Detectors	PMT (Photomultiplier Tubes)
Objective Lenses	Compatible with a wide range of Olympus objectives
Software	Fluoview FV10-ASW software
Scanning Method	Galvanometer
Z-Stack	Yes
Spectral Detection	Yes, with spectral detector option
Weight	Varies depending on configuration
Laser Sources	Multi-line Ar laser (458, 488, 515nm), HeNe(G) laser (543nm), HeNe(R) laser (633nm)

Summary

System Startup Procedure

Image Acquisition Settings and Viewer Overview

Lambda Scanning for Spectral Imaging

Configure spectral imaging settings using lambda scanning.

Microscope Control and Z-Series Setup

Manage objective selection, focus, and z-series acquisition.

Timelapse Imaging Configuration

Set parameters for timelapse imaging, including interval and number.

Imaging Mode Selection

Choose from various imaging modes such as XY, XYZ, and time-lapse.

Image Detection Channel Setup

Configure detection channels and associated settings for image acquisition.

Sample Viewing and Focusing

Image Acquisition Process

Detector Channel Setting Adjustments

Adjusting detector brightness, gain, and offset for optimal image acquisition.

Z-Series Image Acquisition for 3 D Visualization

Sequential Scanning Mode for Cross-Talk Prevention

Line vs. Frame Sequential Scanning

Differentiate between line-by-line and frame-by-frame sequential scanning modes.

Saving and Exporting Images

Saving Images in OIB Format

Save acquired images with full metadata in Olympus Image Binary format.

Exporting Images and Metadata

Export images to various formats and save acquisition metadata.

Image Export Options

File Type and Output Format Selection

Choose export file types like AVI or TIFF and select output formats (RGB, grayscale).

ROI Overlay and Frame Rate Settings

Configure Region of Interest overlays and frame rates for exported movies.

System Shut-Down Procedure