Olympus FV1000 - Image Acquisition Process; Detector Channel Setting Adjustments

OlympusFV1000Userguide August3,2011
D.ImageAcquisition
Clickoffthelightsourcebuttontoturnoff
thenon‐laserlight.
ClickonDyelist buttonanddoubleclick
onthefluorescentdyesyouwanttouse(e.g.
FITCandTRITC)fromthelist.ClickApply
buttonb(ifAssignDyeManuallybuttoncis
checked,uncheckbeforepressingApply
button).Itwillactivatethelaserlinesandset
detectionchannelsupaccordingtothedyes
youselected.

ChooseImagesize(default‐512x512)from
Sizepanel d.

Pressbutton(ifnotpressed)to
automaticallyadjustHVandOffsetvalues
accordingtothescanspeedchange.Click
buttontoscanthesample.Itwill
scanfastat2us/pixelandshowlow‐quality
(pixelated)imageinaLiveViewwindow.

Whilescanning,focusontotheregionofinterestwiththeremotefocuscontrollerorbyclickingarrowhead
buttonsinthemicroscopewindow(largearrowheadmovetheobjectivebytheextentsetbyStepSizee,
andthesmallarrowheadsmovestheobjectivebythehalfofthelargearrowheadstepsize).
ClickStopbutton tostopscanning.

SettheLaseroutputlevel
f(settheMulti‐Arlaser
to1‐5%andtheHeNelaserto1‐25%,depending
onyoursamplecondition,butnolowerthan1%).

Setthescanspeedg to10~12.5us/pixelandclick
XYrepeat buttontoscan(youcanscan
imagefasterorslower,dependingonyoursample).

Detector(CHS)ChannelSetting
:Adjustthe
brightnessbyHV(andGain)andbackgroundblack
level(Offset)ofindividualchannels.KeeptheHV
valuebelow700(beyond700,thebackground
noisewillshowup).

 
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