Olympus Fluoview FV1000 - Setting the ND Filters

Getting Started FLUOVIEW/Outline of LSM Observation Procedures
OPERATION INSTRUCTIONS
1-26
Page
1-2-6 Setting the ND Filters
When you use the laser combiner, you can set the laser intensity by setting ND filter on the
laser combiner.
Display the [Acquire] panel.
Set each laser intensity by sliding the scale bar in the [Laser Intensity] group box of the
[Lasers] sub-panel, in accordance with specimen’s brightness, fluorescence crosstalk and
photo-bleaching.
Fig. 1-7 [Lasers] Sub-panel
While using the HeNe green laser, try out the laser power 50% by setting the [Intensity] scale
bar in the [Laser Intensity] group box .
For other lasers, try the laser power 5% .
[Laser Intensity] group box
Set the laser intensity value
by the scale bar..
The number of the displayed
laser intensity sliders varies
depending on that o
f
channels setting for the
acquisition.