Olympus Fluoview FV1000 - Differences in Image Acquisition Method between Fluorescent and Transmitted; Images; Monochrome Image

APPLIED OPERATIONS/Image Acquisition
OPERATION INSTRUCTIONS
2-61
Page
2-2-3 Differences in Image Acquisition Method Between Fluorescent and Transmitted
Images
2-2-3-1 Monochrome Image
The wavelength obtained by monochrome dyeing can be acquired and observed as an
image of a channel (either Ch1, Ch2, Ch3 or Ch4).
The subsequent description deals with the differences in operation between the
monochrome dyeing and dual-fluorochrome dyeing.
z Set the dyeing method
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Dyes]
sub-panel.
Fig. 2-20 [Dyes] Sub-panel
2. Select the specimen dyeing method in the [Available Dyes] list box in the
[Selected Dyes] group box, and drag the selected method into the field
immediately above the list box.
3. Click the <Apply> button to apply the selected dyeing method to the [Ch] group
box on the upper part of the [Acquire] panel.
When the dyeing method is selected from the [Available Dyes] list box and the
<Apply> button is clicked, a channel for acquiring fluorescence is set
automatically according to the changed filter. And the dyeing method is shown
in the [Ch] group box.
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Apply> button
A
pplies the dyeing method
dragged in the [Selected
Dyes] group box to the
[Ch] group in the [Acquire]
panel.
<Prev.> button
Sets the dyeing method which was
set last time by clicking the
<Apply> button.
Place the pointer on the icon
displayed in the [Selected Dyes], and
the dyeing method is shown in the
pop-up displa
y
.
TIP
[Assign dyes manually]
check box
Checking this enables
the manual setting.
Dragging the dyeing
method in the list directly
to the [Ch] group box
assigns the dye to the
desired channel.