Olympus Fluoview FV1000 - 1 Getting Started FLUOVIEW

CONTENTS
1 Getting Started FLUOVIEW 1-1
1-1 Basic Operations .............................................................................1-1
1-1-1 Microscope .................................................................................................... 1-1
1-1-2 General Mouse Operation Procedures.......................................................... 1-7
1-1-3 Names of Major Panel and Window Controls and Their Functions............... 1-8
1-2 Outline of LSM Observation Procedures ......................................1-9
1-2-1 Turning Power On ....................................................................................... 1-11
1-2-2 Focusing on the Specimen.......................................................................... 1-12
1-2-2-1 Combination with BX .......................................................................................1-12
1-2-2-2 Combination with IX81 FVF .............................................................................1-14
1-2-3 Setting the LSM Light Path.......................................................................... 1-16
1-2-3-1 Combination with Upright Microscope (BX).....................................................1-16
1-2-3-2 Combination with Inverted Microscope (IX81 FVF) .........................................1-18
1-2-4 Selecting the Dyeing Method ...................................................................... 1-20
1-2-5 Selecting the Filters..................................................................................... 1-23
1-2-6 Setting the ND Filters .................................................................................. 1-26
1-2-7 Setting the Observation Condition............................................................... 1-27
1-2-7-1 Setting the Objective Magnification .................................................................1-27
1-2-7-2 Setting the Zoom Ratio to 1X...........................................................................1-28
1-2-7-3 Setting the Channels .......................................................................................1-28
1-2-7-4 Setting the Highest Scan Speed......................................................................1-29
1-2-7-5 Setting the XY Observation Mode ...................................................................1-30
1-2-7-6 Repeated Scanning Operation ........................................................................1-30
1-2-7-7 Setting the Cross-section to be Observed.......................................................1-31
1-2-7-8 Setting the Area to be Observed .....................................................................1-32
1-2-7-9 Setting a Lower Scan Speed ...........................................................................1-32
1-2-7-10 Stopping Repeated Scanning........................................................................1-33
1-2-8 Acquiring Image ..........................................................................................1-33
1-2-9 Saving Image .............................................................................................. 1-34
1-2-10 Exiting from the Software, Turning Power Off ........................................... 1-35