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Olympus U-RFLT50 User Manual

Olympus U-RFLT50
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INSTRUCTIONS
REFLECTED
FLUORESCENCE
SYSTEM
FOR CKX41
A X 7 3 8 9
This instruction manual is for the Olympus Reflected Fluorescence System for
use with the CKX41 culture microscope. To ensure the safety, obtain optimum
performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the system.
Retain this instruction manual in an easily accessible place near the work desk
for future reference.
Modules described in this manual
CKX-RFA
U-LH50HG
U-RFLT50
CKX-NU
This publication is printed on 100% recycled paper

Questions and Answers:

Olympus U-RFLT50 Specifications

General IconGeneral
ModelU-RFLT50
CompatibilityOlympus BX series microscopes
ApplicationFluorescence microscopy
Wattage50 W
Voltage100-240 V

Summary

Safety and Precautions for Reflected Fluorescence System

Essential Safety Guidelines

Essential safety guidelines for operating the fluorescence system and handling its components.

Initial System Setup and Safety

Understanding Safety Symbols and Warnings

Explanation of system symbols and warning indicators for safe operation.

Getting Ready for Operation

Initial steps and precautions before using the system.

System Maintenance and Cautions

Maintenance and Storage Procedures

Procedures for cleaning, maintenance, and proper storage of the system components.

General Usage Cautions and Hazards

Important warnings regarding system usage and potential hazards.

System Component Identification

Nomenclature: System Component Labels

Identification and labeling of major components of the reflected fluorescence system.

Controls of Each Module

Fluorescence Illuminator and Lamp Housing Controls

Detailed description of controls for the fluorescence illuminator and lamp housing.

Power Supply Unit Specifications

U-RFLT50 Power Supply Unit Overview

Overview of the power supply unit features and controls.

Reflected Fluorescence Observation Procedure

Observation Procedure Summary

Step-by-step guide for performing reflected fluorescence observation.

Using System Controls and Precautions

General Precautions for Observation

Key precautions to ensure proper observation and system longevity.

Reflected Light Fluorescent Mirror Selection

Selecting Fluorescent Mirrors for Excitation

Guidance on choosing and using fluorescent mirrors for different excitations.

Applicable Fluorescence Objectives

Fluorescence Objectives List

List of compatible objectives for fluorescence and phase contrast microscopy.

Mercury Burner Ignition and Startup

Turning On the Mercury Burner

Procedure for igniting the mercury burner and initial stabilization.

Mercury Burner Centering

Centering the Mercury Burner Arc

Step-by-step guide to correctly center the mercury burner arc.

Field Iris Diaphragm Adjustment

Centering the Field Iris Diaphragm

Procedure for centering and adjusting the field iris diaphragm for optimal contrast.

Filter Slider Operations

Switching the Filter Slider

Instructions on how to use the filter slider for ND filters and shutter.

Troubleshooting Guide

Optical System Troubleshooting

Common problems and solutions related to the optical path of the system.

System Specifications

Specifications and Operating Environment

Technical details and specifications for system modules and operating environment.

System Assembly Procedures

Assembly Diagram

Visual guide illustrating the step-by-step assembly of the system components.

Detailed Assembly Procedures

Removing Dummy and Dust Covers

Steps to remove dummy cover, dust covers, and fluorescent mirror slider.

Removing Fluorescent Mirror Slider

Procedure to detach the fluorescent mirror slider from the illuminator.

Attaching System Components

Attaching Fluorescence Illuminator

Step 3: Attaching the CKX-RFA reflected fluorescence illuminator.

Attaching Fluorescent Mirror Slider

Step 4: Attaching the fluorescent mirror slider.

Attaching Dust Covers

Step 5: Attaching the dust covers to the system.

Final Assembly Steps

Attaching Light Shield and Lamp Housing

Steps 6 & 7: Attaching the excitation light shield and U-LH50HG lamp housing.

High-Pressure Mercury Burner Installation

Attaching the Mercury Burner

Procedure for securely installing the mercury burner into the lamp housing.

Optional U-Excitation Filter Set

CKX-NU Filter Set Components

Information about the optional U-excitation filter set and its components.

Power Supply Cord Connection

Proper Selection of Power Supply Cord

Guidelines for selecting and using the correct power supply cord.

Fuse Replacement

Fuse Replacement Procedure

Steps for safely replacing fuses in the power supply unit.

Certified Power Cord Agencies

Table 1: Certified Cord Agencies

List of agencies and their certification marks for power supply cords.

HAR Flexible Cord Marking Standards

Table 2: HAR Flexible Cord Marking

Details on HAR flexible cord harmonization marking methods.

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