Pall Allegro - User Manual

Questions and Answers

K
Kristin StuartAug 14, 2025
Why is the single-use mixer system snagged during inflation in Pall Laboratory Equipment?
- A
  Alex SchneiderAug 14, 2025
  The single-use mixer system might be snagged during inflation because the regulated pressure setting is being reached in the mixer biocontainer, or due to misalignment of the powder port in the support arm. Check that the single-use mixer system has not become stuck during the inflation process. Also, check that the powder port is fully located within the support arm such that the LED is illuminated. Additionally, a malfunction of the micro switch could be the reason. Check the cable connection to the micro switch. If problems persist, contact Pall.
E
Eric WestAug 17, 2025
What to do if the ‘Door’ LED on the Pall Laboratory Equipment UCI is NOT illuminated GREEN?
- A
  Amy HoldenAug 17, 2025
  If the ‘Door’ LED on the UCI isn't green, first, make sure the door is fully closed and the handle is in the closed position to activate the micro switch. If it still doesn't light up, check the cable connection to the micro switch. If problems persist, consider replacing it or contacting Pall for support.
Z
zoliverAug 20, 2025
What to do if the ‘Shaft’ LED on the Pall Allegro UCI is NOT illuminated GREEN?
- M
  Marc KimAug 20, 2025
  If the ‘Shaft’ LED on the UCI isn't green, verify the system is correctly connected to the power supply. If the issue persists, inspect the cable connection to the micro switch. Replace the micro switch or contact Pall for assistance if needed.
L
Lauren MasonAug 25, 2025
What to do if the ‘Support’ Interlock LED on the Pall Allegro Laboratory Equipment UCI is NOT illuminated GREEN?
- T
  Todd PayneAug 25, 2025
  If the ‘Support’ Interlock LED isn't green, first check that the system is correctly connected to the power supply. If the problem continues, check the cable connection to the micro switch. Also, check the micro switch is working correctly by manually depressing the switch. Ensure the single-use system is correctly installed, aligning the shaft and seal housing assembly so that the LED illuminates and the seal housing is flush with the tube support flange. If problems persist, contact Pall.
M
Matthew AguilarAug 27, 2025
Why won't the motor run on my Pall Laboratory Equipment?
- W
  William CookAug 27, 2025
  If the motor of your Pall Laboratory Equipment isn't running, there are a few potential reasons. First, the rechargeable battery may not be charged; in this case, plug the power cord into an electrical outlet and charge the battery for 10 hours. Second, the peak load protection might be activated. To resolve this, reduce the load to the stated maximum capacity and reset the main switch by turning it off and then back on. Finally, the main switch may have popped out; simply push the main switch button back in.
N
nelsonkaitlynAug 30, 2025
What to do if the rechargeable battery of Pall Allegro cannot be charged?
- J
  James RochaAug 30, 2025
  If the rechargeable battery of your Pall Laboratory Equipment cannot be charged, it could be due to a couple of reasons. First, there might be no power from the electrical outlet. Try changing to a different outlet. Second, the rechargeable battery itself might be defective, in which case you should replace it.
S
Sheila PhillipsSep 3, 2025
What to do if the battery status display is off on my Pall Laboratory Equipment?
- H
  Heather FaulknerSep 3, 2025
  If the battery status display on your Pall Laboratory Equipment is off, it could be due to the main switch being off; push the main switch button in. Alternatively, the battery or battery status display could be defective, and you should have the device repaired by the manufacturer.
P
phelpsmichaelSep 6, 2025
Why is the loading platform moving too slowly on my Pall Allegro?
- L
  Lauren GarciaSep 6, 2025
  If the loading platform of your Pall Laboratory Equipment moves too slowly, it is likely that the rechargeable battery is weak. In this case, have the device repaired by the manufacturer.
R
Richard SparksSep 9, 2025
How to fix the buzzer when it fails to sound on the tape switches of Pall Allegro Laboratory Equipment?
- F
  fletcherkevinSep 9, 2025
  If the buzzer on the tape switches of your Pall Laboratory Equipment fails to sound, it could be due to a defective buzzer or defective tape switches. In either case, have the device repaired by the manufacturer.

Summary

Introduction to Allegro 200 L Mixer

Safety Warnings for Allegro Mixer Operation

Receipt and Handling of Allegro Mixer Equipment

Allegro 200 L Mixer System Pictures and Schematics

System Installation and Operation Guide

Connecting to the User Control Interface (UCI)

Connecting power and gas supply lines to the UCI for system setup and initialisation.

Safety Interlocks (micro-switches) Check Procedure

Procedure to verify the correct functioning of safety interlock micro-switches (Shaft, Door, Support) via the UCI.

Pre-Installation Checks for Mixer Tote and Single-Use System

Steps to prepare the mixer tote and inspect the single-use system components before installation.

Allegro 200 L Mixer System Installation

Detailed instructions for physically installing the Allegro single-use system into the mixer tote and securing it.

Mixer Bag Inflation Process

Guidance on inflating the mixer biocontainer, including gas connection, pressure monitoring, and support arm attachment.

Adding Fluid and Solids to the Mixer System

Procedures for connecting fluid supply and adding liquid and solid materials to the mixer biocontainer.

Operating the Allegro 200 L Mixer

Instructions for controlling mixer speed, direction, starting, stopping, and understanding operational limits.

Drain and Removal Instructions for Mixer System

Troubleshooting Common Allegro Mixer Issues

Allegro 200 L Mixer Specification Tables

General Maintenance Recommendations for Allegro Mixer

WEEE Compliance, Reuse, Recycling, and Recovery

Allegro Mixer System Warranty Information

CE Declaration of Conformity for Allegro 200 L Mixer

The Allegro™ 200 L Single-Use Mixer is a device designed for mixing solutions in biopharmaceutical applications. It consists of a mixer tote and a single-use biocontainer, providing a sterile and efficient mixing solution.

Function Description

The Allegro 200 L Single-Use Mixer is used for preparing and mixing solutions in a controlled environment. The system utilizes a single-use biocontainer within a reusable mixer tote, which minimizes cleaning and validation efforts between batches. The mixer operates by inflating the biocontainer and then using an impeller to mix the fluid. It supports both fluid addition and draining operations, with options for maintaining homogeneity during dispensing.

Important Technical Specifications

Mixer Tote (LGRMXTTE200L230 / LGRMXTTE200L120):

Voltage: 230 Vac (for LGRMXTTE200L230) or 120 Vac (for LGRMXTTE200L120)
Current: 5.1 A (for 230V) or 9.2 A (for 120V)
Frequency: 50 Hz (for 230V) or 60 Hz (for 120V)
Volume: 50 – 200 L
Gas Supply: 4 – 6 barg
Gas Connection: 10 mm Pneumatic tubing outside diameter
Weight: 120 Kg (without fluid in system)
Footprint: 1054 mm (W) x 748 mm (D)
Height: 1378 mm
Materials of Construction (Mixer Tote): 304 SS

Mixer Biocontainer:

Materials of Construction:
- Biocontainer film; outlet flange; inlet manifold and top hat: Low Density Polyethylene (LDPE)
- Impeller: Polysulfone (PS)
- Outlet port: Polysulfone (PS)
- Shaft: Stainless Steel (SS)
- Outlet O-rings: Silicone

Motor:

Power: 0.37 kW

UCI Details (LGRMXUCI200L230 / LGRMXUCI200L120):

RPM: 50 – 200

Usage Features

Installation and Operation:

Receipt of Equipment: Upon receipt, users should inspect the hardware for damage and ensure all components are present. The mixer hardware should be stored in an appropriate indoor location, clean rooms, and classified areas.
System Installation: The system involves connecting the power cord to the User Control Interface (UCI) and ensuring proper gas supply connections. Safety interlocks (micro-switches) are crucial for operation, including those for the shaft, doors, and support arm.
Biocontainer Installation: The single-use system is securely placed into the mixer tote, aligning the impeller shaft with the tube support and inlet port plate. The locating plate on the outlet port must be correctly positioned.
Inflation and Exhaust Tubing: The biocontainer is inflated using the gas inlet, and exhaust tubing is connected to the UCI. The inflation process is monitored via a pressure gauge.
Closing Doors: The mixer tote doors are closed and secured, ensuring the system locating plate remains under the closed doors. The handle is turned to the 'closed' position, and the 'DOOR' LED on the UCI should illuminate green.
Locking Doors: The doors are locked using a key for security.
Support Arm Activation: The support arm micro switch is depressed, and the 'SUPPORT' LED on the UCI should illuminate green, indicating proper engagement of the powder port.
Baffles: The mixer tote can be installed with baffles to improve mixing efficiency and reduce air entrapment and vortex generation.
Fluid Addition: Fluid is added to the single-use mixer system through the fluid inlet line. The pressure in the biocontainer should be monitored during filling, ensuring it does not exceed 20 mbar.
Powder Addition: Powder can be added once the desired volume of fluid is achieved. The bag inflation control valve is switched to 'OFF' position, and the powder port is opened for adding solids.
Operating the Mixer: The mixer speed (RPM) and direction of rotation (clockwise/downflow or counter-clockwise/up-flow) can be controlled via the UCI panel. The maximum continuous mixing life is 3 days (72 hours).

Drain/Removal Instructions:

Draining: The outlet port is connected to downstream equipment, and the outlet port valve is opened to allow fluid to flow out. Draining can be performed with the mixer off (for stable solutions) or running (to maintain homogeneity).
Gas Blanket: If a gas blanket is used during draining, it must be deactivated at the end of dispensing.
Fluid Recovery: The tote design maximizes fluid recovery, with typical residual volumes less than 150 mL. Lifting the edges of the biocontainer can further improve recovery.
Biocontainer Removal: After drainage, all inlets and outlets are closed and tubing removed. The tote doors are unlocked and opened. The impeller shaft is pulled out, and the biocontainer is fully removed for disposal according to local regulations.

Maintenance Features

General Maintenance Recommendations:

Annual Servicing: It is recommended that the Allegro 200 L single-use mixer be serviced on an annual basis by Pall Advanced Separation Systems (PASS).
Critical Spares: Certain critical spares should be kept in stock to prevent system downtime. These include:
- Support arm/seal housing microswitch (200 L mixer tote) (Part Number: DC201772C00)
- Door magnetic switch (200 L mixer tote) (Part Number: DC201773C00)
- UCI cabinet key (Part Number: DC201774B00)
- Tote/lock key (Part Number: DC201775B00)
- Tube support assembly (Part Number: KDC201340C00)
- Support arm (Part Number: DC200694C00)
- Motor and gearbox assembly (Part Number: DC201385C00)
- UCI (230 V) (Part Number: LGRMXUCI200L230)
- UCI (120 V) (Part Number: LGRMXUCI200L120)
Optional Spares:
- Baffle kit (Part Number: LGRMXBK200L)
- Powder bag support frame (Part Number: LGRMXPBS200L)

Troubleshooting: The manual provides a detailed troubleshooting guide for various issues, including:

'Shaft' LED not illuminated: Possible causes include the system not being powered up or a malfunction of the micro switch. Corrective actions involve checking power supply, cable connections, and replacing spare items.
Misalignment of seal housing: Re-installing the single-use system with careful alignment of the shaft and seal housing.
'Door' LED not illuminated: Check power supply, ensure the door is closed, and the handle is in the 'closed' position.
'Support' LED not illuminated: Check power supply, cable connections, and ensure the powder port is fully located.
Inflation process stopped: Check if the single-use mixer system is snagged.

WEEE Compliance: The product contains electrical and electronic materials and should be disposed of separately from unsorted waste, in accordance with European Directive 2002/96/EC, to prevent adverse environmental and health effects. Users are advised to recycle the product when replacing it or when it has outlived its useful life.

Warranty: Pall warrants that Allegro hardware and systems, when properly stored, installed, and operated according to specifications, will be free from defects in material and workmanship during their shelf life. Pall's liability is limited to replacing or crediting defective Allegro systems and hardware.