Pall T Series User Manual

Safety Conventions in this Manual
Safety information is identified in this instruction manual by the following convention:
Information
Information: Identifies important information about the current topic.
Caution
Caution: Identifies a situation that may cause product damage and may pose a safety risk that can
cause personal injury.
Warning
Warning: Identifies a dangerous or potentially dangerous situation that may cause irreversible damage to
equipment and poses a safety risk that can cause serious personal injury.
1. Introduction
1.1 Applications in TFF
Tangential Flow Filtration (TFF) is an efficient method for concentrating, desalting, or exchanging
buffer solutions of biomolecules ranging in volume from several milliliters to thousands of liters.
It can be used to fractionate large from small biomolecules, depyrogenate buffer solutions,
harvest cell suspensions, and clarify fermentation broths and cell lysate.
TFF is utilized to perform various steps on a wide range of applications in the biopharmaceutical
industry, such as:
• Concentration and desalting of solutions of proteins, peptides and oligonucleotides
• Purification and recovery of antibodies or recombinant proteins from cell culture media
• Clarification of cell lysate or tissue homogenates
• Recovery of products expressed into the media from cell culture
• Preparation of samples (concentrate, desalt, or buffer exchange) before or after
column chromatography
Caution: This guide is designed only for T-Series cassettes with Omega membrane. Its contents
and specifications do not apply to other Pall tangential flow filtration membrane cassettes.
1.2 Tangential Flow Filtration Process
The process conditions for using membrane cassettes in a Tangential Flow Filtration system
depend on the specific application. Important process parameters that must be considered
include:
• Retentate flow rate (cross flow rate)
• Transmembrane pressure
• Temperature
• Product characteristics (such as concentration, viscosity, and additives)
Variations in any of these parameters can affect the quality and reproducibility of the TFF
process.
The operating conditions for any TFF process must be established by performing trials,
evaluating results, and then modifying conditions as necessary to achieve the required results.
Because tangential flow filtration membrane cassettes and assemblies are used in repeated
processing modes, proper care and use is crucial to ensure reliable and reproducible
performance from run to run, and to maximize the life of the membrane cassettes.
This manual contains detailed steps for the proper installation, preparation, cleaning and
storage of a tangential flow filtration (TFF) membrane cassette and assembly. The procedures
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Pall T Series Specifications

General

Brand	Pall
Model	T Series
Category	Laboratory Equipment
Language	English