Pall T Series - Table of Contents

Contents
Safety Notice ..........................................................................................................................4
Learn About Safety ...............................................................................................................5
Important — Read First ........................................................................................................5
Safety Conventions in this Manual .......................................................................................6
1. Introduction .......................................................................................................................6
1.1 Applications in TFF ........................................................................................................6
1.2 Tangential Flow Filtration Process ..................................................................................6
1.3 The TFF Process ..........................................................................................................7
1.4 System Hardware Configurations ..................................................................................8
1.4.1 Options ................................................................................................................8
1.4.2 System Design ....................................................................................................9
1.4.3 Ports and Flow Rate ............................................................................................9
2. T-Series Cassettes with Omega Membrane Installation .................................................9
2.1 First-time Use of Cassette Holder/TFF System ..............................................................9
2.1.1 Flush and Clean the System ................................................................................9
2.1.2 Making a Flushing Gasket ..................................................................................10
2.1.3 System Flushing Protocol ..................................................................................10
2.2 Installing T-Series Membrane Cassettes with Omega Membrane into Autotorque (AT)
and Manual Torque (MT) Holders ................................................................................11
2.2.1 Preparation for Loading Cassettes ....................................................................11
2.2.2 Unpacking the Cassettes ....................................................................................11
2.2.3 Installing Cassettes and Gaskets ......................................................................13
2.3 How to Torque Pall AT and MT Cassette Holders ......................................................14
3. Operating Specifications ................................................................................................17
3.1 Recommended Cross Flow Rates for T-Series Cassettes with Omega Membrane ......17
3.2 Operating Pressures, Temperatures and pH ................................................................18
3.3 Hold-up Volume and Minimum Working Volume ........................................................18
3.3.1 Determining Feed/Retentate Hold-up Volume ....................................................18
3.3.2 Determining Permeate Hold-up Volume ............................................................19
3.3.3 Determining the Non-recoverable Volume ..........................................................20
3.3.4 Determining the System Minimum Working Volume ..........................................20
4. Preconditioning T-Series Cassettes and Systems .........................................................21
4.1 Using a Cassette for the First Time ............................................................................22
4.2 Initial Flushing of the Cassette and Assembly (WFI Flush) ............................................22
4.2.1 Flush the Feed/Retentate and Permeate Line to Waste ....................................22
4.3 Sanitizing and Depyrogenating the Cassette and Assembly ........................................23
4.3.1 Add and Recirculate Sanitizing Solution ............................................................24
4.4 Flushing the Cassette and Assembly after Sanitization ................................................24
4.4.1 Flush the Retentate and Permeate Line to Waste ..............................................24
4.4.2 Water Recirculation ............................................................................................25
4.4.3 Flush the Retentate and Permeate Line to Drain ................................................26
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