Pall T Series User Manual

7www.pall.com/biopharm
include protocols for Pall T-Series screen channel cassettes with Omega membrane.
Some processes may require adaptations to suit the specific requirements of a given system
configuration or application, but essential protocols should not be omitted.
To perform these procedures, it is recommended that the TFF assembly include 3 pressure
gauges/transducers and 2 valves for proper execution of the protocols presented,
see Figure 2. To include integrity testing capability (highly recommended), additional valves may
be required (Figure 22).
1.3 The TFF Process
The steps required in a Tangential Flow Filtration process are displayed in Figure 1.
Preconditioning and post-use conditioning are covered in Sections 4 and 5 of this manual.
Information on product process optimization and processing can be found in other supporting
literature, at www.pall.com or through Pall Life Sciences Technical Support:
• PN33289 Diafiltration: A Fast Efficient Method for Desalting or Buffer Exchange of Biological
Samples
• PN33213 Introduction to TFF for Laboratory and Process Development Applications
Figure 1
The TFF Process
Pre-use
Conditioning
Processing
Post-use
Conditioning
1. Installation
2. Flushing
3. Sanitization
2. Flushing
4. Normalized Water
Permeability (NWP)
5. Integrity Test
6. Buffer
Conditioning
8 and 9.
Concentration
and/or
Diafiltration
10. Product
Recovery
11. Buffer Flush
12. Clean In
Place (CIP)
2. Flushing
4. Normalized
Water Permeability
13. Storage
7. Optimization
(Once only)

Pall T Series Specifications

General

Brand	Pall
Model	T Series
Category	Laboratory Equipment
Language	English