PerkinElmer Lambda 365 - Page 20

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a. Y Unit: Select one of the display unit: Absorbance, Transmittance, Reflectance or Energy.
b. SBW (nm): Select bandwidth. There are five bandwidths selectable: 0.5, 1, 2, 5 or 20 nm.
c. Tm Method: Select a method for determining melting temperature. Options include: 1st
derivative and Average.
d. Equation Name:
Enter the name of the formula. %G-C is entered as default, of which equation is formulated in
Equation Expression.
e. Equation Expression
The default equation for the calculation of %G-C base pairs is:
%G-C=2.44 * (Tm - 81.5 - 16.66 * log(M) + 500/K)
Where M is the molarities of salt in mol/L, K is the DNA base pair length. %G-C will be
calculated with estimated T
m
and input values, Molarities (M) and length of DNA (K).
f. DNA Pair Length (K)
Enter the DNA base pair length. This value will be used calculating %G-C.
g. Calculation Start Temperature (°C)
Enter the start temperature for calculating the T
m
value.
h. Calculation End Temperature (°C)
Enter the end temperature for calculating the T
m
value.
i. Molarity (mol/L)
Enter the salt molarities of the solution [mol/L]. This value will be used in calculating %G-C.
7. After setting parameters for Experiment and Lamp, click the Accessory tab. And select Multi-
Cell Peltier.