PSI FluorPen FP 100 User Manual

38
9.F. Light Curve (LC) Protocol
The protocol called Light Curve (LC) was designed to acquire parameters for construction of Light
Response Curve relating the rate of photosynthesis to photon flux density. The method is based on
successive measurements of the sample exposed to a stepwise increase of light intensity. The effective
quantum yields of photosynthesis are determined under various light intensities of continuous
illumination. Measurement is based on pulse modulated fluorometry (PAM).
Several LC protocols are predefined in FP. These differ in number and duration of individual light phases
and light intensities.
# of phases
Phase duration
Light intensities, [mol m
-2
s
-1
]
LC1
6
60s
10; 20; 50; 100; 300; 500
LC2
5
30s
100; 200; 300; 500; 1000
LC3
7
60s
10; 20; 50; 100; 300; 500; 1000
The protocol includes following measured and calculated parameters:
Abbreviation
Explanation
Fo
minimum fluorescence in dark-adapted state
Fm
maximum fluorescence in dark-adapted state
Fm_Ln
‡
maximum fluorescence in light adaptation state
Ft_Ln
‡
instantaneous fluorescence during light adaptation
QYmax*
maximum quantum yield of PSII in dark-adapted state - Fv/Fm
QY_Ln
‡
**
instantaneous PSII quantum yield induced in light
‡
n represents a sequential number of light phase
* Calculated as (Fm – Fo) / Fm
** Calculated as (Fm_Lx – Ft_Lx) / Fm_Lx

PSI FluorPen FP 100 Specifications

General

Brand	PSI
Model	FluorPen FP 100
Category	Measuring Instruments
Language	English