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R-Biopharm KOBRA CELL - User Manual

R-Biopharm KOBRA CELL
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R-Biopharm Rhône Ltd
R-Biopharm – for the safety of your analysis.
KOBRA® CELL Art. No. K01
Instruction Manual
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R-Biopharm KOBRA CELL Specifications

General IconGeneral
BrandR-Biopharm
ModelKOBRA CELL
CategoryMeasuring Instruments
LanguageEnglish

Summary

Introduction to the KOBRA CELL

Intended Use

Describes the primary applications and purpose of the KOBRA® CELL in HPLC analysis for aflatoxin detection.

Derivatisation Reaction Mechanism

Explains the electrochemical process involving bromine generation for aflatoxin derivatisation and its importance for detection.

KOBRA CELL Contents and Safety

Package Contents

Lists all the components included in the KOBRA® CELL package, such as the cell, power pack, and tubing.

Required Reagents

Details the specific reagents (e.g., Distilled Water, Methanol, Potassium Bromide, Nitric Acid) needed for operation.

Accessory Products

Information on optional or complementary products available for use with the KOBRA® CELL system.

Handling Hazards

Outlines the hazardous nature of mycotoxins and necessary precautions, including protective clothing.

Decontamination Procedures

Provides instructions for safely decontaminating labware and waste contaminated with hazardous substances.

KOBRA CELL Assembly

Components Identification

Identifies and illustrates the individual components that make up the KOBRA® CELL assembly.

Assembly Precautions

Key safety and handling instructions to ensure correct and leak-free assembly of the KOBRA® CELL.

Step-by-Step Assembly

Detailed instructions and visual guidance for the physical assembly of the KOBRA® CELL unit.

KOBRA CELL Installation and Operation

Power Pack Information

Notes on the KOBRA® CELL power pack, its design, and plug conversion for different regions.

Power Pack Technical Specifications

Details the technical specifications of the power pack, including voltage, current, and battery life.

Installation Guide

Diagrammatic representation of how the KOBRA® CELL connects within the HPLC system.

Installation Instructions

Step-by-step guide for connecting the KOBRA® CELL, mobile phase, and tubing within the HPLC setup.

Pre-Operation Precautions

Essential safety measures and checks to perform before initiating operation of the KOBRA® CELL.

Operation Instructions

How to turn the KOBRA® CELL power pack on and off for system operation.

Error Indicator Light

Explanation of the LED indicator on the power pack and the conditions that trigger the error light.

HPLC Conditions for Analysis

KOBRA CELL Maintenance

Storage Guidelines

Procedures for safely storing the KOBRA® CELL, including keeping the membrane wet.

Daily Cleaning Procedure

Step-by-step instructions for daily cleaning to maintain the KOBRA® CELL and HPLC system.

Performance Monitoring

Methods for regularly checking KOBRA® CELL performance to detect membrane deterioration.

Membrane Replacement

Instructions on when and how to replace the KOBRA® CELL membrane for continued optimal performance.

Troubleshooting Common Issues

No Peaks Observed

Diagnoses potential causes when no peaks appear on the HPLC chromatogram, beyond the KOBRA® CELL.

Reduced or Missing Aflatoxin Peaks

Troubleshooting steps for low or absent peaks of Aflatoxin B1 and G1, checking connections and membrane.

Abnormal Peak Shape

Addresses issues related to abnormal peak shapes, such as poor resolution or tailing, often related to the column.

Power Pack Error Light

Explains why the error light on the power pack illuminates and checks to perform.

Leakage Troubleshooting

Addresses potential causes of leakage from the KOBRA® CELL, primarily related to screw tightness.

High Pressure Issues

Troubleshooting steps for when the KOBRA® CELL causes high pressure readings on the HPLC system.

Storage Plug Issues

Guidance for issues with broken or lodged storage plugs, recommending professional repair.

General Information and Support

Product Quality Standards

Details the quality management systems (ISO 9001, ISO 13485) under which RBR products are developed.

Technical Support Services

Lists the support services offered to customers, including problem analysis and installation assistance.

Warranty Information

Outlines the warranty terms and limitations for R-Biopharm Rhône Ltd products.