18
R-Biopharm Rhone Ltd
7
Troubleshooting
7.1 No Peaks Observed
• If no peaks are observed on the HPLC
chromatogram it is probably not the KOBRA® CELL
that is at fault (Fig. 8). There may be a problem
with a component of the HPLC system or with the
standards used.
• Check that the bulb in the lamp does not need
replaced. It should be replaced every 1,000 - 2,000
hours depending on the detector.
• Check there is not a problem with the detector.
• Check the standard has been prepared correctly,
or the standard has not deteriorated. Standards
should be prepared fresh every day.
• Check the PEEK
TM
tubing leading to and from the
KOBRA® CELL is not blocked or distorted. Replace if
required.
• Check the internal components of the
KOBRA® CELL have been inserted in the correct
order, particularly if no peaks are observed
following the replacement of one of the
components (Fig. 1).
Fig 8. No Peaks Obtained in Chromatogram
To Next Page
Loading...
Need help?
General