R-Biopharm KOBRA CELL - Reduced or Missing Aflatoxin Peaks

19
R-Biopharm Rhone Ltd
7.2 Reduced / No Aflatoxin B1
and G1 Peaks Observed
• Check the KOBRA® CELL is connected correctly to
the HPLC (Fig 6).
• Check the connections to the KOBRA® CELL from
the power pack.
• Check the KOBRA® CELL membrane. The
membrane is the site of the actual derivatisation
reaction (ion-exchange occurs on the membrane).
The membrane starts a deep amber colour and,
through time and use, the centre of the membrane
becomes discoloured, eventually turning a pale
colour. The pale colour indicates that the
membrane will need replaced.
• Check there is power in the power pack. Check
that the power pack has been turned on.
• Check the internal components of the
KOBRA® CELL are in the correct order (Fig. 1), or
that there is not a blockage in the system.
• No potassium bromide (or not enough potassium
bromide) was added to the mobile phase. Prepare
again.
• No nitric acid was added to the mobile phase.
Prepare again.
Fig 9. Reduced / No Aflatoxin B1 and G1 Peaks Observed