R-Biopharm KOBRA CELL - HPLC Conditions for Analysis

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R-Biopharm Rhone Ltd
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HPLC Conditions
HPLC Conditions
Derivatisation KOBRA® CELL at 100 µA setting
Guard Cartridge Inertsil ODS-3
5 µm, 4 mm x 10 mm or (Hichrom) equivalent
Analytical Column Inertsil ODS-3V
5 µm, 4.6 mm x 150 mm (Hichrom) or equivalent
Mobile Phase Water : Methanol (60 : 40 v/v)
Add 119 mg of potassium bromide and 350 µl 4 M Nitric Acid to 1 litre of mobile
phase
HPLC Pump To deliver mobile phase
Flow Rate 1.0 ml/minute
Fluorescence
Detector
Excitation: 362 nm
Emission: 425 nm (B1 and B2)
455 nm (G1 and G2)
Column Heater Maintain guard and analytical columns at 40 °C
Integrator / Data
Control System
From preferred supplier
Injector Autosampler / Reodyne valve
Injection Volume 100 µl
Elution Order G2, G1, B2, B1.