Sanyo MCO-5M - Connection of N; Gas Cylinder

75
19
BEFORE COMMENCING OPERATION
Connection of N
2
(or O
2
) gas cylinder
WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of gas. Elevated level of gas can be hazardous to health and may lead to
asphyxiation and risk of death.
This incubator needs N
2
or O
2
gas depending on the setting of O
2
density. The selection is as follows:
When the setting of O
2
density is less than 18%: N
2
gas
When the setting of O
2
density is more than 22%: O
2
gas
O
2
density in the atmosphere is about 20%. For the control of O
2
density in the chamber, O
2
gas is
diluted by N
2
gas when the setting of O
2
density is less than that of the atmosphere. On the contrary, O
2
gas is added when the setting of O
2
density is more than that of the atmosphere.
1. Install a pressure regulator (optional accessory MCO-100L) on N
2
or O
2
gas cylinder. Or use a
regulator rated at 25MPaG (250kgf/cm
2
G, 3600psiG) on the primary side and 0.2MPaG (2.0kgf/cm
2
G,
30psiG) on the secondary side.
2. Using the gas supply tube provided, connect the pressure regulator to the N
2
or O
2
inlet (A/B) located
at the rear left side of the incubator. Connect the main cylinder to the inlet A and connect the
sub-cylinder to the inlet B so that the gas supply can be switched automatically when one gas cylinder is
empty. Connect the same gas type to both inlet A and B. Never connect N
2
gas and O
2
gas at the
same time to the inlet A and B respectively.
3. Set the N
2
or O
2
gas pressure on the secondary side to 0.05MPaG (0.5kgf/cm
2
G, 7.1psiG) (at gas
injection). The higher gas pressure makes control range of O
2
density wider. In addition, excessive
pressure may cause disconnection of internal pipes inside the incubator, which will result in leakage of N
2
or O
2
gas into the atmosphere. Elevated level of CO
2
gas or O
2
gas can be hazardous to health and
may lead to asphyxiation and risk of death or fire by gas leakage. The repair of the incubator will be
necessary if the internal pipe is disconnected.
4. Check that no gas is leaking at any point where the pipe connects with the gas regulator or the
incubator.
Note:
• Refer to “Procedure for replacement of gas cylinder” enclosed with the unit at the time of replacement.
• The incubator, including the gas supply pipes and services must be examined at frequent intervals to
ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.
WARNING
O
2
gas increases the susceptibility of substances to burn. Take care of the handling of flame in a room
where the incubator is installed.