6.0 Frequently Asked Questions |17
6.0 Frequently Asked Questions |17
6.0 Frequently Asked Questions
6.1 No movement
•
Check Scientifica logo is illuminated on the front of the rack
• Check for correct switch position on side of cube for controlling
manipulator
• Check all cables and connectors for damage or loose connections
6.2 Drift
•
Allow the system to warm up
• With most electrical components on the rig needing a warm-up
period, it is good practice to allow a certain warming-up time
after switching on the power of the set-up (up to one hour).
• Check all bolts are fully tightened
• Is the drift detected with the perfusion running?
(test for longer than 10 minutes)
• Perfusion solution with a temperature different to the room
can lead to thermal expansion of the pipette – leave some
time for the pipette to adjust to new temperatures.
• Fast perfusion may cause drift of the pipette.
• Ensure the vacuum source is strong enough to break the
surface tension of the fluid in the bath
• Is the microscope firmly mounted to the table top?
• Many microscopes have rubber feet underneath. These may
degenerate with time and cause the microscope to be
unstable. Ideally the microscope should be bolted down onto
the table top.
• Is the cooling headstage switched off?
• Some amplifiers have a cooling facility on the headstage
(which is normally located on the amplifier front or back
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