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Scientifica MDU User Manual

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Multiphoton
Detection Unit (MDU)
Setup and operation manual
Revision 1.0
Manual Part No.: S-MDU-MANUAL

Table of Contents

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Scientifica MDU Specifications

General IconGeneral
BrandScientifica
ModelMDU
CategoryLaboratory Equipment
LanguageEnglish

Summary

1.0 Introduction

1.1 Handling Scientifica equipment – precautions

Important safety information related to general use of the Scientifica MDU.

1.2 The Scientifica Multiphoton Detection Unit (MDU)

Describes the Scientifica Multiphoton Detection Unit (MDU) and its configurations.

2.0 Packing List

2.1 Standard items

Lists standard components included with the MDU for various configurations.

3.0 Mechanical setup

3.1 Fitting the above-stage MDU to the SliceScope

Instructions for attaching the above-stage MDU to the SliceScope microscope frame.

3.2 Above-stage variant (fixed objective)

Describes the setup for the above-stage variant with a fixed objective.

3.3 Above-stage variant manual with manual objective changer (in vivo)

Describes the manual objective changer option for rapid objective interchange.

3.4 Above-stage variant with Motorised Objective Changer (MOC)

Details the MOC variant for automatic objective changes, typically for RMS-thread objectives.

3.5 Substage variant

Describes the setup for the substage variant using a high-NA oil-immersion condenser.

3.6 Filter Cube Installation

Explains the use and installation of filter cubes for spectral response and two-channel detection.

4.0 Optical System Preparation

4.1 Transmission Viewing

Recommendations for transmission viewing, including Köhler illumination and dichroic mirror use.

4.2 Transmitted Laser Imaging

Using transmitted IR laser radiation for imaging and generating reference images.

4.3 Laser Beam Delivery

Details optics for imaging scanner mirrors onto the objective lens for efficiency and resolution.

4.4 Control Software and Data Acquisition

Information on compatible control software and data acquisition.

5.0 Electrical setup

5.1 Electrical setup - controller connections

Instructions for connecting the controller unit, including power and detector module cables.

6.0 Operation

6.1 Powering up the system

Safety routine and steps for powering up the system for the first time.

7.0 Troubleshooting

Dichroic mirror actuator jams.

Troubleshooting steps for a jammed dichroic mirror actuator, likely due to foreign bodies.

No activity on the controller (meters and logo both fail to light).

Steps to diagnose issues with the controller, including power supply and connections.

No high-voltage reading on either detector regardless of "enable" switch position or voltage controls.

Diagnosing lack of high voltage, checking interlocks, connectivity, and cables.

No high-voltage reading on one detector whilst the second detector is fully functional.

Troubleshooting for a single detector not showing high voltage, checking interlocks and contacts.

No apparent signal coming from a detector (but control voltage apparently operational).

Diagnosing lack of detector signal, checking data acquisition and connectivity.

Excessive noise and offset during imaging at low detector gain (voltages between 0V and -700 V)

Identifying causes of noise and offset at low detector gain, such as stray light.

Excessive noise during imaging at high detector gain (voltages between -800V and -1100V)

Troubleshooting excessive noise at high gain, detector overload, or dark current.

Variable noise and offset down an image.

Addressing variable noise and offset, often due to visible light entering the laser beam path.

Smearing or "trails” behind narrow, bright objects along scan lines, narrow features appear too wide (horizontally).

Diagnosing smearing or trails in images, often due to scan speed exceeding detector bandwidth.

Excess noise in digitized signals (especially compared to the oscilloscope trace)

Troubleshooting excess noise in digitized signals, often caused by under-sampling.

Low or no multiphoton signal, or unusually large laser power required to obtain any signal.

Diagnosing low signal or high laser power requirements, checking spot intensity and aberrations.

Shading at extremes of field, shading on one side or other, shading at top or bottom

Addressing image shading issues, often related to scanner occlusion or objective alignment.

Loss of signal with depth

Explains signal loss with depth due to scattering and reduced laser intensity.

Limited field of view (FoV) in multiphoton images

Discusses limited field of view in images due to scanning too wide or deep.

8.0 Specifications

8.1 Above-Stage Variant with fixed objective and U-AAC condenser

Provides dimensional specifications for the above-stage variant with fixed objective.

8.2 Above-Stage MOC Variant with U-AAC condenser

Details specifications for the above-stage MOC variant, including objective compatibility.

8.3 Above-Stage Fixed Objective Variant with Substage variant

Describes specifications for a four-channel system, useful for thick tissue slices.

8.4 Above-Stage Variant with MOC and Substage Variant

Provides specifications for a combined MOC and substage variant system.

9.0 Appendix:

9.1 Removing and Replacing a Detector Module

General procedure for removing and replacing detector modules for maintenance or repair.

9.2 Detector Module removal

Detailed steps for safely removing a detector module from the optical block.

9.3 Detector Module Replacement

Instructions for inspecting the optical block and PMT module before re-assembly.

10.0 Warranty, Technical Queries and Returns

11.0 About Scientifica

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