Page 7 of 58
1.2.2 Product Walkthrough
Figure A: System Overview
Objective fittings
• A wide range of objectives can be fitted directly to the MDU block,
including M32 X 0.75, M27 X 0.75, M25 X 0.75 and RMS thread.
Alternatively, a motorised Objective Changer can be connected for use with
RMS-thread objectives (such as an X4 and an X40 NA 0.8 Water-
Immersion lens). An optional manual objective changer is available to
permit the use of low-power objectives for setting up and larger, higher-
power objectives for imaging.
Pre-fitted and aligned dichroic mirror which transmits laser radiation
(wavelength longer than 665 nm) through the optical block but which
intercepts and directs visible fluorescence from the sample towards the
detectors. This dichroic mirror can be withdrawn from the optical path by a
safe imaging switch on the front of the optical block, leaving clear access to
the objective.
IR laser blocking filter (excludes scattered laser radiation at wavelengths
longer than 680 nm from the detectors).
Within U-
MF2 –
includes
8a, 8b &
7
An un-populated filter cube is provided, but it need not be used if only one
detection channel is required.
Separator dichroic - enables simultaneous detection in two different
wavebands for experiments using more than one fluorophore. An un-