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3.2 Above-stage variant (fixed objective)
Please remember: adjustment or removal of any screws or components other than those
noted in this manual can adversely affect the performance and operation of your equipment
and may invalidate your warranty.
Note that the nominal experiment height is 219 mm above the base of the microscope unless
otherwise stated.
3.2.1 Checks and preparation
Check the input dichroic mirror:
o If it is not already there, move the silver actuator lever on the front of the optical block to the left
(anti-clockwise). Look into the top entry port: you should have a clear view to the bottom port
(which should be capped by a white plastic transit cover). A black shutter plate should be
evident at the back of the input port covering the collection optics.
o Move the silver actuator lever on the front of the optical block to the right (clockwise) whilst
looking down into the top entry port. The lever should move freely and pull into its final position.
You should see the dichroic mirror move into position with the mirror in place, and the transit
cover on the lower port will no longer be visible through the dichroic mirror.
If the dichroic mirror carriage does not move freely, refer to the troubleshooting
section.
If the dichroic mirror appears cracked or is apparently absent, contact Scientifica for
assistance.
If the dichroic mirror is dusty, refer to the maintenance section for cleaning
procedures.
o Move the dichroic mirror out of the beam for safety by rotating the silver actuator anti-clockwise
(left). This is the safe (default) position to which the control should be returned whenever
imaging is not underway.
Unscrew the white plastic transit cap from the lower entry port of the optics block. Retain this cap for
shipping and storage. The threaded hole in which this cap was fitted (M32 X 0.75) is designed to
accept the microscope objective (possibly via a supplied adapter).
Remove any parts that are fitted in the circular dovetail mount at the front of the SliceScope top plate
so that there is clear access through the hole.
Using the SliceScope motorised controls, adjust the upper Z-focus drive to its central position. The
central position is reached when the top of the moving block is level with the top of the static back-
plate.
3.2.2 Fitting Objectives
For optimum optical performance, it is recommended that microscope objectives be attached to the lower
casing of the optical block (using appropriate adapters). This will ensure that the detectors can capture light
from the extreme edges of the objective’s field of view and at the maximum range of angles – both of which
are of special importance when imaging in deep, scattering samples. The fitting is also light-tight.
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