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3.3 Above-stage variant manual with manual objective changer (in vivo)
Figure H: In vivo variant with manual objective changer
Users might wish to change objectives in a more rapid and convenient manner (perhaps using a lower-power
objective during setting up, and a higher power objective for imaging). A manual objective-changing
mechanism is available for this purpose.
The slider has magnets in it which help with correct alignment and retention of the objective / slider assembly
against the optical block, and which also support the weight of the objective. The slider presents a threaded
hole identical to that in the base of the optical block: it can accept objectives directly or it can accept any of the
supplied adapters. Two sliders are supplied so that you can swap between two favourite objectives rapidly.
One configuration is worthy of particular note: if you want to use a very-wide-field, high-NA medium power
objective (such as x 20 NA 1.0 or x 25 NA 1.1) for imaging, but your camera / tube lens combination does not
give sufficient field of view to permit convenient setting up, then you may wish to fit the second slider with a
very low power (e.g. X4) air objective on the extended RMS adapter. This will give a wider field of view whilst
maintaining par-focality with the larger objective. The extended RMS adapter positions the objective 30 mm
below its usual position, and is intended for use with 45 mm parfocal objectives.
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