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condenser lens acting as the primary collection optic instead of a conventional microscope objective. In this
configuration, the MDU is suspended on an X, Y stage which is in turn attached to the condenser focusing
mechanism: this permits the microscope to be adjusted for Köhler illumination in the usual way. The system
allows conventional illumination of the sample by either visible or infra-red light when two-photon imaging is
not in progress.
Above and below stage (simultaneous) configuration
In addition to using above or below stage MDUs independently, both can be applied simultaneously to
optimise fluorescent collection. Both above stage and below stage MDUs can be fitted with one or two
detection modules. This means that up to four channels of signal collection can be recorded simultaneously.
It is also possible to gain access to the transmitted laser radiation that has passed through the sample: this
can be used to form an image using a suitable (photo-diode) detector.
Two controllers are required for this four-channel system, giving fully independent control of each channel’s
gain settings.
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