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Are the stops on the rotary stage set correctly?
• Magnetic Lock: When setting the approach angle, ensure the lower lock stop
position of the vertical rotation lock is used. The magnetic lock should ensure that
the sliding bracket doesn’t rotate downwards whilst freely standing.
• Screw Lock: Make sure that the clamping screw is tightened when in the
recording position.
For more information on setting the rotary stage stops, see section 3.2.3
Are the stops on the rotary base set correctly?
• Magnetic Lock: Make sure that the manipulator stops on the magnetic lock
position when swung into the recording position, you will hear the magnet catch
indicating the micromanipulator is in the correct position.
• If there is a small amount of tension on a cable, the PatchStar can move away
from the lock during the experiment.
• If the strength of the Magnetic lock is not strong enough contact Scientific.
For more information on setting the rotary stage stops, see section 3.2.3
Is the Headstage or probe holder attached correctly?
• Make sure that the headstage is firmly attached
• Be careful that you don’t over tighten the clamp, because this may cause
expansion and may also be a source of drift.
• Check that the headstage-mounted sliding bracket is clamped at the end of its
travel.
Is the Headstage grounding wire supported ?
• Make sure that the headstage is firmly attached. Be careful that you don’t over
tighten the clamp as this may cause expansion and may also be a source of drift.
Is the pipette holder attached firmly and the O-ring new and of appropriate size?
• With pipettes being exchanged very often, there is a lot of wear on the O-ring
inside the pipette holder which may lead to it not holding the pipette firmly.
• Also make sure that there is no tension on the pipette suction tube
• Ensure that the AgCl wire inside the pipette is not touching the front end of the
electrode.
Are the 3 linear stage wires and the rotation sensor wires supported?
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