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INSTRUCTION MANUAL
2019
COMPLETING THE TRANSECT
1. When you have reached your desired sample volume or the end of the transect
distance, quickly rotate the pole to invert the filter housings.
2. Continue walking at the transect speed until all water is filtered and tubing line
is cleared.
3. You may need to isolate suction to each filter housing to effectively dry them
all. This can be done by pinching the suction tubing of the other two filters,
thus directing all suction pressure to a single filter housing. Repeat this process
for all three filters.
4. Preserve the filters according to the preservation procedure outlined above.
5. When recording the sample volume, divide the Liters value on the display by
the number of filters used in the transect to determine sample volume per filter
replicate.
STERILIZATION
By design the eDNA Sampler system does not need to be sterilized between each
sample. However, when moving between watersheds, it may be advisable to ster-
ilize the system to avoid transferring bacteria or pathogens between sensitive
systems. The eDNA sampler can be sterilized using the following procedure.
1. Fill a reservoir (e.g. 1L bottle) with a 2% bleach water solution. This can be
achieved by adding 20ml of household bleach to a 1L bottle and filling it the
rest of the way up with tap water. Note: Higher concentrations of bleach may
damage internal components.
2. Place both the IN and OUT tubes (without filter) into the bleach water and
start the pump.
3. Allow the pump run and circulate the bleach water for 10 minutes, then stop
the pump.
4. Rinse the system with clean tap water by repeating the process using only tap
water.
CLEANING
The sampling pump can be cleaned with a soft cloth and a mild soap and water
solution. Do not use chlorinated cleaners on the window as these will damage the
window. Do not use solvent based cleaners as these may damage the integrity of
the enclosure.
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