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Smith-Root eDNA Sampler - Sample Preservation Techniques; Filter Folding and Vial Placement; Sample Labeling and Field Storage; Metadata Recording

Smith-Root eDNA Sampler
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13
INSTRUCTION MANUAL
2019
SAMPLE PRESERVATION
1. For immediate sample preservation, open the filter housing by pulling on the
nozzle tab which will expose the filter membrane.
2. Fold the filter using the forceps provided in filter packet and place into the vial
containing user-provided preservative (e.g. ethanol). Note: the nozzle extension
tube can be used to help the filter folding process (see above).
3. Label the sample with an ID and place the preserved sample in a storage box.
Samples can then be stored in the dry bags of the eDNA Sampler unit.
4. Record the Log ID from the interface associated with the sample ID.
5. Record any other metadata associated with the sample (weather, water temp,
etc.).
6. Move on to next sampling location.
Steps for folding the
filter and placing in
vial.
1. Open filter housing
2. Extract filter using
forceps included in
kit.
3. Use nozzle exten-
sion end to aid
folding filter.
4. Insert filter into
vial.
5. Firmly close cap on
vial.