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SPEX 270M - User Manual

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270M
Rapid Scanning Imaging
Spectrograph/Monochromator
User Manual
Including:
Graphical Monochromator Interface
Software
and
SPEX.SYS Device Driver
Part Number 80121
Leica Microsystems, Inc.'s Ex. 1033
Page 1 of 89
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SPEX 270M Specifications

General IconGeneral
Monochromator TypeCzerny-Turner
Wavelength Range200 - 800 nm
Grating Grooves1200 grooves/mm
DetectorPMT (Photomultiplier Tube)
Entrance Slit Width10 - 2000 μm
Exit Slit Width10 - 2000 μm

Summary

About the Manuals

Overview of the 270 M

Total Automation

Details on automated moving parts, control options, and remote operation capabilities.

Rapid Scanning Wavelength Drive

Describes the high-speed sine drive for grating movement and its benefits for data acquisition.

Optical Design Optimized for Imaging

Explains the Czerny-Turner configuration and its optical correction for imaging performance.

Dual Axis Grating Turret

Discusses the optional 220TK grating turret and its automated grating interchange capabilities.

Unpacking

Inspection for Damage

Procedure for checking the instrument for visible damage upon delivery.

Initial Setup

Instructions for installing leveling legs and familiarizing with instrument components.

Removal of Shipping Restraints

Guidance on carefully removing shipping restraints from optical components.

Environmental Considerations

Recommendations for operating the spectrometer in a controlled atmosphere and temperature.

Electrical Connections

Power Connection

Details on connecting the instrument to an AC power source.

Control Cable Connections

Information on cable connections for controlling the 270M based on interface type.

Shutter Cable Connections

Instructions for connecting shutters (225MCD or 226MCD) to the detector control.

Software Installation and Control Interfacing

Standard Graphical Monochromator Interface Software (GMI)

Guide to installing and using the GMI software for system control.

SPEX.SYS Driver

Details on the low-level driver for controlling the spectrometer via custom programs.

Optional HandScan

Information on using the HandScan controller for system operation.

Optional SPEX232 and SPEX488 interfaces, and DataScan Controller

Setup procedures for optional interfaces and the DataScan controller.

Optional DM3000 Spectroscopy Computer

Instructions for controlling the 270M with the DM3000 Spectroscopy Computer.

Optional AutoScan Software

Guide to setting up and using the AutoScan software.

Optional QuikScan PDA system

Instructions for controlling the 270M with a QuikScan PDA system.

PDALOAD.EXE Driver

Information on the PDALOAD.EXE driver for use with QuikView software.

Controlling the 270 M with the Standard Spectrometer Interface

Details on interfacing directly using TTL logic with the standard I/O.

Basic Operation

Explains the basic operational states and signal transitions for controller interaction.

Signal Descriptions

Defines the SELECT, DIRECTION, STEP, INDEX, and SENSE signals.

Accessory Installation

270 MCA Spectrograph Exit Port Installation

Procedure for installing the spectrograph exit port for array detectors.

225 MCD Shutter

Installation guide for the external mount shutter at the entrance slit.

226 MCD Shutter

Instructions for installing the internal mount shutter for exposure control.

Installation and Care of Optical Components

Mirrors and Gratings

Maintenance advice for mirrors and gratings, including cleaning procedures.

Single Grating Mounts

Instructions for installing and removing the standard 220G single grating mounts.

Turret Grating Mounts

Guidance on using and potentially removing the 270TK interchangeable grating turret.

Entrance and Exit Slits

Description of motorized adjustable slits and their control.

Spectrograph Exit Port

Details on the 270MCA adapter for mounting array detectors.

Initial Checks

Equipment Required

List of necessary equipment for performing initial checks and calibration.

Checkout

Procedures for verifying system functionality and calibration using test lines.

Optical Interfacing Considerations

Grating Selection

Factors to consider when selecting gratings for specific applications and performance.

Optical Coupling at the Entrance Slits

Tips for optimizing light coupling into the entrance slit for maximum throughput.

Exit Slit Considerations

Analysis of exit slit width's effect on bandpass and throughput.

Spectrograph Port Focusing

Procedure for setting up array detectors and achieving optimal focus and alignment.

Mechanical Interfacing

Application Examples

As a Monochromatic Illuminator

Using the 270M to provide a specific bandpass and tune wavelength.

As a Scanning Monochromator

Measuring spectral output by sequentially presenting wavelengths to a detector.

As a Simple Spectrograph

Simultaneously detecting spectral signals over a range of wavelengths.

As an Imaging Spectrograph

Detecting multiple spectra from light imaged at various heights in the entrance slit.

Specifications

Alignment

Equipment Required

List of equipment needed for alignment procedures.

Grating Adjustment

Procedures for adjusting gratings, including wavelength centering and vertical alignment.

Wavelength Centering Adjustment

Steps to center the wavelength line using adjustment screws on the grating mount.

Grating Vertical Adjustment

Procedure to ensure the image is vertically centered at the exit slit.

Rocking the Grating

Method to correct tilted diffraction planes by rocking the grating.

In Case of Difficulty

Troubleshooting

Common issues and solutions for spectrometer problems like no response or partial response.

Service Policy

Return Authorization

Requirements and procedures for returning instruments or components for service.

Removing returnable components from your instrument

Instructions for safely removing gratings and circuit boards for return.

Appendix A: 270 M AC Power Selection and Fusing

Appendix B: 270 M Interface Connector Pin Assignments

Appendix C: Spectrometer Control Board Data

Appendix D: Circuit Board Drawings

Appendix E: SPEX.SYS Device Driver, Version 1.1

Installing the SPEX.SYS device driver

Guide to installing the SPEX.SYS driver in the DOS CONFIG.SYS file.

SPEX0000 Command Protocol

Details the ASCII command structure for controlling the spectrometer.

Moving a device

Explains relative and absolute step move functions for device positioning.

Device Calibration

Procedure for calibrating device positions within the driver's internal table.

To Query a Device

Function to retrieve the current step position of a requested device.

To Query Number of States

Determines the number of system states controlled by the device driver.

Reading the Device

Method for retrieving information stored in the driver's internal buffer.

Hardware Table Specification

Table specifying hardware numbers, devices, frequencies, and regulation.

Power On Settings for the 270 M

Describes initial device states and movements upon system power-up.

Communicating with the SPEX.SYS Driver

Methods for communicating with the driver via C, GWBasic, text files, and DOS command line.

Communicating from a Text File

Using DOS COPY command to send commands from a text file to the driver.

Communicating from the DOS Command Line

Sending commands directly from the keyboard via the DOS command line.

Appendix F: Program Examples for SPEX.SYS

Sample BASIC Program

Example BASIC program demonstrating control of the 270M via SPEX.SYS.

Sample Pascal Program

Example Pascal program illustrating control of the 270M via SPEX.SYS.

Sample C Program

Example C program showing control of the 270M via SPEX.SYS.