Why is stirring speed important for TA Instruments Nano ITC Measuring Instruments?
A
Angela Butler MDAug 4, 2025
Stirring speed is important because, generally, a stirring speed of 250 to 400 rpm is appropriate for instruments made with gold sample and reference cells. In Hastelloy cell instruments, the best results are often obtained with stirring speeds of 150–200 rpm. Higher speeds generally result in higher noise levels in the baseline. Stirring too slowly may result in broad titration peaks that require longer intervals between injections.
L
Linda ReevesAug 12, 2025
How to minimize blank corrections in TA Instruments Nano ITC?
A
Andrew ElliottAug 13, 2025
To minimize blank corrections:
* The ligand (titrant) solution should always be made up in the same dialysis buffer used for the protein (titrand).
* If the ligand is also a protein, it should be dialyzed in the same buffer.
* Less concentrated solutions also have lower dilution heats and should be used when possible.
E
Erica BakerAug 23, 2025
How to operate TA Instruments Nano ITC at non-ambient temperatures?
S
sean97Aug 23, 2025
When operating at non-ambient temperatures, it is important to allow adequate time between injections for the titrant to equilibrate to the calorimeter temperature before injection. Thorough degassing of the titrant is especially important when operating above the ambient temperature.
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