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TA Instruments Nano ITC Series User Manual
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Revision F Issued F
ebruary 2011
Getting Started Guide for
Models 5300, 5301, 5302
Nano Isother
mal
Titr
ation Calor
imeter
(Nano ITC)
2
Table of Contents
Introduction
3
Important: TA Instruments Manual Supplement
3
Notes, Cautions, and Warnings
4
Regulatory Compliance
4
Safety Standards
4
Safety
5
Electrical Safety
5
Lifting the Instrument
5
Table of Contents
7
Chapter 1: Introducing the Nano ITC
9
Overview
9
Instrument Models Covered in this Guide
9
The Nano Isothermal Titration Calorimeter (ITC)
10
Applications
10
Batch/Incremental Titration
10
Titration/Data Analysis
11
Calculation of Equilibrium Constants
11
System Components
12
Measuring Unit
12
Reaction Vessel
13
Syringe/Stirrer
14
Buret Assembly
15
Options and Accessories
15
Instrument Specifications
16
Chapter 2: Installing the Nano ITC
17
Unpacking/Repacking the Nano ITC
17
Installing the Instrument
17
Inspecting the System
17
Choosing a Location
18
Near
18
Away from
18
Power Requirements
18
Setting up the Nano ITC
19
Connecting the Cables and Cords
19
Starting the Nano ITC
20
Shutting down the Instrument
21
Chapter 3: Use, Maintenance, & Diagnostics
23
Overview
23
Calibrating Chemicals
24
Heat of Protonation of Tris Base
24
Sample Preparation
24
Experiment Setup
24
Experiment Parameters
24
Preparing and Degassing the Solutions
27
Degassing Solutions
27
Preparing the Sample and Reference Cells
28
Loading the Buret
30
Installing the Buret Assembly
31
Starting the Experiment
32
Cleaning the Nano ITC
33
Analyzing the Data
36
Maintaining the Nano ITC
37
Purging the Nano ITC
37
Troubleshooting the Nano ITC
38
Minimizing Blank Corrections
38
Operating at Non-Ambient Temperatures
38
Stirring Speeds
38
Stable Instrument Operation
39
Appendix A: Buret Position Functions
41
Index
43
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TA Instruments Nano ITC Series Specifications
General
Brand
TA Instruments
Model
Nano ITC Series
Category
Measuring Instruments
Language
English
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