VICI M50 - Page 17

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6: Action line
A step in the method sequence, reflecting the values entered by selecting
ortypingvaluesinthePump,Valve,andWaitelds.Tomovetoadiffer-
ent line for editing, just click on it or use No. 12: Up and Dn buttons.
7: Pump function
Aspirate: Drawliquidinto the line connected to the pump outlet(PortB).
Dispense: Force a pre-programmed amount of liquid through the pump.
Wash: Wash or flush the lines (always in the outward direction,
or A to B) with system fluid or a pre-programmed reagent
selected.
8: Volume
The amount to be aspirated, dispensed, or sent through as a wash.
9: Flow rate
The rate at which the given volume is aspirated, dispensed, or sent
through as a wash.
10: Wait field
If a value is entered in this field for an action line, during a run the
program will wait for the designated time (in seconds) after the
completion of that action before advancing to the next action. Used
after an aspirate or dispense step to allow the liquid to equilibrate in the
line or the dispensed liquid to come to equilibrium, as with viscous
liquids or very small capillary tubing.
11: Event box
When this box is clicked when programming an action line, the program
will stop before that action is executed. The program will remain stopped
until No. 4: Event Button is clicked. (If the step before the event line
has a programmed Wait interval, the Event button will not trigger the
action until the Wait interval has elapsed.)
12: Up and D(ow)n buttons
In the Editing mode, these move the line highlighter up and down.
13: Valve type
 Multi-Pos: Multipositionstreamselector
2-Pos: Twopositionswitchingvalve/injector
14: Valve Position
The position the valve needs to be in to select the proper solvent or
reagent.
15: Valve Direction(forMulti-Posonly)
The direction the valve will take to the indicated position – the shortest
(used in most cases), clockwise, or counterclockwise.
16: Insert button
Inserts a new action line above the highlighted line.
17: Duplicate button
 Duplicatesthehighlightedline.Thenewlinecanthenbeeditedasrequired.
18: Delete button
 Deletesthehighlightedline.
19: End Edit button
When editing is complete, click this button to return to the Idle mode,
ready to Run.